转多个抗真菌蛋白基因水稻植株的获得及其抗稻瘟病菌的初步研究  

Introduction of Multiple Antifungal Protein Genes into Rice and Preliminary Study on Resistance to Pyricularia oryzae of Transgenic Rices

作　　者：冯道荣[1] 卫剑文[1] 许新萍[1] 许耀[1] 李宝健[1] 

机构地区：[1]中山大学生物工程研究中心

出　　处：《中山大学学报（自然科学版）》1999年第4期62-66,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家863计划资助项目

摘　　要：用基因枪法将水稻碱性几丁质酶（ＲＣ２４）基因、苜蓿葡聚糖酶（βＧｌｕ）基因、大麦核糖体失活蛋白（ＢＲＩＰ）基因和潮霉素（ｈｐｔ）基因同时导入籼稻品种（七丝软占）中，获得了７个潮霉素抗性再生系，Ｓｏｕｔｈｅｒｎｂｌｏｔ证明２～３个抗真菌蛋白基因已整合到水稻基因组中．Three antifungal protein genes: the rice basic chitinase gene, the alfalfa glucanase gene, the barley ribosome inactivating protein gene and a hygromycin phosphotransferase gene were introduced into an indica variety riceQisiruanzhan by biolistic Seven independent lines were obtained by selecting for hygromycin resistance Southern blot proved two or three antifugal protein genes integrated into rice genome The transgenic plants showed an enhanced resistance to Pyricularia oryzae in vitro

关 键 词：转基因水稻 抗真菌蛋白基因 基因枪 抗稻瘟病菌 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S511.034[农业科学—植物保护]