2种虾虎鱼细胞色素b基因全序列克隆与分析  

Cloning and sequence analysis of Cyt b gene in 2 Gobiidae fishes

作　　者：陈雷[1] 程远志[1] 王日昕[1] 徐田军[1] 

机构地区：[1]浙江海洋学院海洋科学学院,海洋生物资源与分子工程实验室,浙江舟山316000

出　　处：《南方水产》2010年第5期43-49,共7页South China Fisheries Science

基　　金：国家科技支撑计划项目(2007BAD43B08);浙江省重大科研项目(2007C02001)

摘　　要：测定了矛尾刺虾虎鱼（Acanthogobius hasta）和红狼牙虾虎鱼（Odontamblyopus rubicundus）的线粒体细胞色素b（Cytb）基因全序列。2种虾虎鱼Cytb基因序列全长均为1141bp,通过序列比对,检测到266个变异位点,序列中碱基转换频率高于颠换,碱基替换主要发生在密码子第3位。结合GenBank中的虾虎鱼科其他物种的细胞色素b基因全序列,利用Kimura-双参数模型分析遗传距离表明,10种虾虎鱼科鱼类的遗传差异在0.118~0.330之间,大头新虾虎鱼（Neogobius kessleri）与同属的裸喉新虾虎鱼（N.gymnotrachelus）遗传距离最小,为0.118;大头新虾虎鱼和矛尾刺虾虎鱼遗传距离最大,为0.330。以Cytb基因全序列构建的NJ进化树分析表明,10种虾虎鱼分为3个主要类群,红狼牙虾虎鱼和矛尾刺虾虎鱼聚为1支,且与其余8种虾虎鱼类遗传距离较远。The complete sequences of Cyt b genes in Acanthogobius hasta and Odontamblyopus rubicundus were amplified,cloned and sequenced.The Cyt b genes in both species are 1 141 bp in length.Comparing the obtained sequences,we detected 266 nucleotide variation sites,finding more transitions than transversions.Most base substitutions occur at the 3rd locus of the codon.Based on the complete Cyt b sequences of Gobiidae fishes recorded in GenBank,the genetic distance among 10 Gobiidae species calculated by Kimura-2 parameters model ranges from 0.118 to 0.330.The distance between Neogobius kessleri and N.gymnotrachelus is the shortest,being 0.118,while the distance between N.kessleri and A.hasta is the farthest,being 0.330.The phylogenetic analysis based on Cyt b gene sequence using NJ method suggests that the 10 Gobiidae fishes are divided into 3 main groups.Specifically,O.rubicundus and A.hasta cluster into one clade,having a relative far genetic distance to the other 8 Gobiidae species.

关 键 词：虾虎鱼科 细胞色素B 序列分析 系统发生 

分 类 号：Q532.8[生物学—生物化学]