Analytical Behavior of Fura-2 and Its Determination of [Ca2+]i in Lymphocytes Treated with Cefotaxime  

作　　者：双少敏 乔晓娜 王丹丹 樊丽 芦冬涛 慕丽晓 周叶红 

机构地区：[1]School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China [2]China Research Institute of Daily Chemical Industry, Taiyuan 030006, China

出　　处：《Journal of Measurement Science and Instrumentation》2010年第4期395-400,共6页测试科学与仪器（英文版）

基　　金：supported by the Key Project of National Nature Science Foundation of China(NO.90813018);the Returned Chinese Scholars Fund

摘　　要：The interaction of Fura-2 with Ca^2＋ is studied using steady fluorescence technique. The effect of pH on the spectra behavior of Fura-2 in the presence of Ca^2＋ is investigated, the excitation maxima （340 nm） and the isobestic point （360 nm） for the fluorescence spectra of Fura-2 depend on pH. At different temperatures the apparent dissociation constants （ Kd ） of Fura-2-Ca^2＋ complex are examined, Kd is found to decrease with increasing temperatures （20 ℃, 37 ℃, 50 ℃ ） and △His calculated to be 21.16 kJ/mol by using the Van＇ t Hoff equation at pH 7.4 for all the temperatures tested. The determination of intracellular Ca^2＋ concentration （ [Ca^2＋ ] i ） in lymphocyte is developed by using Fura-2 as a fluorescence probe in the presence of Cefotaxime at 37 ℃ and pH 7.4.

关 键 词：FURA-2 pH temperature [Ca^2＋]i CEFOTAXIME 

分 类 号：R96[医药卫生—药理学]