重度子痫前期和正常妊娠胎盘中微小RNA-155及富含半胱氨酸蛋白61的差异表达  

Different expression of microRNA-155 and cysteine-rich 61 in human placentas from severe preeclamptic and normal pregnancies

作　　者：王志群[1] 苏莉[1] 刁振宇[1] 周建军[1] 戴毅敏[1] 张艳青[1] 胡娅莉[1] 

机构地区：[1]南京大学医学院附属鼓楼医院妇产科,210029

出　　处：《中华围产医学杂志》2010年第5期403-407,共5页Chinese Journal of Perinatal Medicine

基　　金：基金项目：国家自然科学基金（30872775）

摘　　要：目的探讨微小RNA-155（microRNA-155，miR-155）和富含半胱氨酸蛋白61（cysteine—rich61，CYR61）在重度子痢前期和正常妊娠胎盘中差异表达的意义。方法取18例重度子痫前期患者（孕36～40周）胎盘和同期分娩且孕周匹配的18例正常产妇胎盘，从mRNA水平检测miR-155和CYR61mRNA的表达情况，并在蛋白水平检测CYR61的表达情况。结果与正常产妇胎盘相比，重度子痢前期组胎盘的miR-155表达水平明显增高（165．7±16．4和527．9±49．1，t=7．00，P〈0．01）；而CYR61mRNA表达水平及蛋白表达水平均降低（31．7±2．7和16．4±1．2，t=5．10，P〈0．01；36．4±1．5和19．7±1．2，t=36．26，P〈0．01）。重度子痢前期和正常妊娠胎盘组织中的miR-155与CYR61mRNA的检测结果的相关性分析显示，两组内两者表达均呈负相关（r=-0．52，P〈0．05；r=-0．57，P％0．05）。结论重度子痢前期患者胎盘中miR-155的上调，可能与促血管生成因子——CYR61的表达下降有关。miR-155、CYR61可能共同参与了子痂前期胎盘血管缺陷的发生。Objective To investigate the different expression of mieroRNA 155 （miR-155） and cysteine-rich 61 （CYR61） in human placentas between severe preeclamptic and normal pregnancies. Methods Placentas were obtained from severe preeclamptic and healthy control pregnant women （n= 18 for each group） at 36-40 gestational weeks. The expressions of miR-155 and CYR61 mRNA were assessed by real-time quantitative reverse transcription-polymerase chain reaction, and the levels of CYR61 protein were tested by Western blot. Results Compared with the control group, the miR 155 expression was increased in placentas from severe preeclampsia groups （165. 7 ± 16. 4 vs 527.9±49.1,t=7.00, P〈0.01）, and the CYR61 mRNA expression （31.7±2.7 vs 16.4±1.2,t= 5.10,P〈0.01）, as well as the CYR61 protein expression （36.4±1.5 vs 19.7±1.2,t=36.26, P〈 0.01 ） were decreased. There was a significantly negative correlation between the expression of miR 155 and CYR61 mRNA within both groups （preeclamptic group： r=-0.52, P〈0.05;control： r=-0.57, P〈 0.05）. Conclusions Up-regulation of placental miR-155 in severe preeclampsia may be related to the decreased expression of CYR61. Both miR-155 and CYR61 may contribute to the disorders of placental angiogenesis in severe preeclampsia in human.

关 键 词：妊娠 先兆子痢 微RNAS 富含半胱氨酸蛋白61 胎盘 

分 类 号：R714[医药卫生—妇产科学]