肥大细胞特征检测与盆腔症状评分联合应用——间质性膀胱炎诊断标准研究  

Correlation study on mast cells＇ property and PUF scale in interstitial cystitis

作　　者：王旭[1] 张卫[2] 韩瑞发[2] 

机构地区：[1]北京航天总医院泌尿外科,100076 [2]天津医科大学第二医院泌尿外科 天津市泌尿外科研究所

出　　处：《中华泌尿外科杂志》2010年第9期618-620,共3页Chinese Journal of Urology

摘　　要：目的 探讨间质性膀胱炎（IC）黏膜组织中肥大细胞特征与盆腔症状评分（PUF）联合应用诊断IC的应用价值.方法 IC患者膀胱黏膜活检组织标本18例,12例正常膀胱组织标本作为对照组.标本0.5%甲苯胺蓝染色,光镜下计数肥大细胞密度,连续10个视野取其均数;锇酸液固定,透射电镜下观察肥大细胞与脱颗粒肥大细胞的超微结构;比较2组标本肥大细胞浸润密度、脱颗粒肥大细胞构成比.统计学分析肥大细胞浸润密度、脱颗粒比率与PUF评分的相关性.结果 IC组织标本中肥大细胞密度（28～76个/mm2）明显高于对照组（7～15个/mm2）,差异有统计学意义（Z=3.927,P＜0.01）;IC组织中75.3%的肥大细胞呈不同程度脱颗粒状态;IC患者PUF评分（17～35分）明显高于对照组（0～8分）,差异有统计学意义（t=14.736,P＜0.01）;PUF评分与肥大细胞浸润数量无线性关系（rs=-0.618,P=0.601）.结果 肥大细胞浸润是IC组织特征性的病理表现;IC组织中肥大细胞浸润数量与PUF评分无线性相关关系.联合检测IC组织中肥大细胞浸润密度、肥大细胞激活脱颗粒状态与PUF评分可作为IC诊断的有效指标.Objective To compare the mast cells＇ property and the level of pelvic pain, urgency and frequency （PUF） scale in urinary bladder between interstitial cystitis （IC） patients and controls.Methods Eighteen cystoscopy biopsy specimens of interstitial cystitis patients and 12 controls were stained with 0.5% toluidine blue and immobilizated with Osmic Acid. Then the mast cells were observed and counted with light microscope and transmission electron microscope. The PUF scale and the number of mast cells between the 2 groups were compared. Results The mast cell＇s number of the interstitial cystitis samples（28-76 pieces/mm2） was significantly higher than that of the non IC persons＇ bladder tissues（7-15 pieces/mm2） （Z=3.927,P〈0.01）. 75.3% mast cells were in a state of being activated degranulation. The PUF scale of IC patients（ 17-35 scores）was significantly higher than that of the non IC persons＇ （0-8 scores） （t=14.736,P〈0.01）. The PUF scale of the patient group did not have a linear IC relation with the mast cell＇s infiltrated number among the specimens （rs=-0.618,P=0.601）. Conclusions Mast cell infiltration may be one of the characteristic pathological manifestations of IC. The association of mast cell infiltration and the PUF scale may be a new diagnosis criteria for IC.

关 键 词：膀胱炎 间质性 肥大细胞 参考标准 

分 类 号：R686[医药卫生—骨科学]