恩度调控人脐静脉血管内皮细胞MicroRNAs的表达  

Modulatory effect of Endostar on expression of microRNAs in HUVECs

作　　者：张荣阁[1] 殷铁军[1] 晁腾飞[1] 张珺[1] 凌慧芬[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院综合医疗科,武汉430030

出　　处：《中华实验外科杂志》2010年第10期1442-1444,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察恩度对人脐静脉内皮细胞（HUVECs）中MicroRNAs（miRNAs）表达的影响,探讨恩度通过调控MicroRNAs发挥抗血管生成的作用机制.方法 采用实时荧光定量逆转录-聚合酶链反应（QRT-PCR）技术,检测50、100、200、500μg/L恩度对HUVECs干预前及干预1、2、4、8 h后miR126及miR221表达水平,并用2-△△CT法计算相对表达的倍数变化.结果 miR126、miR221在HUVECs中均高表达 恩度显著下凋miR126的表达（0.07±0.01～0.56±0.04,P＜0.01） 相反,恩度明显上调miR221表达（1.45±0.06～8.87±1.51,P＜0.01） 干预前后,miR126及miR221表达水平差异有统计学意义（P＜0.05）.结论 恩度显著影响HUVECs中miR126、miR221表达,提示恩度存在通过调控MicroRNAs发挥抗血管生成作用的机制.Objective To investigate the effects of Endostar on the expression of microRNAs in the human umbilical vascular endothelial cells （HUVECs） and the anti - angiogenesis mechanism of Endostar by modulating microRNAs. Methods The expression levels of miR126 and miR221, which were interfered with different concentrations of Endostar （50, 100,200,500 μg/L） for different durations （1,2, 4, 8 h）, were detected by real-time fluorescent quantitation reverse transcription-polymerase chain reaction （QRT-PCR）. Relative gene expression data were analyzed by 2 - △△Cr method. Results Over expression of miR126 and miR221 was detected in the HUVECs. The expression of miR126 was down regulated by Endostar significantly （0.07 ±0.01- 0.56 ±0.04,P 〈0.01 ）, as compared with that before interference by Endostar. The expression of miR221 was obviously up - regulated by Endostar （ 1.45 ± 0.06 -8.87 ±1.51 ,P 〈 0.01 ）, as compared with that before interference by Endostar. Conclusion Endostar can regulate the expression of miR126 and miR221 in the HUVECs, suggesting that Endostar exerts its anti - angiogenesis by modulating microRNAs.

关 键 词：MICRORNAS 恩度 抗血管生成 肿瘤 

分 类 号：R965[医药卫生—药理学]