麻疯树干旱诱导差异表达基因片段的分离与功能分析  

Cloning and function analysis of drought related gene fragment with differential expression in Jatropha. curcus

作　　者：戴晓[1] 苟春宝[1] 王勇[1] 杨彩兰[1] 陈巍[1] 陈放[1] 魏炜[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川大学学报（自然科学版）》2010年第5期1189-1194,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家"十五"科技攻关(2002BA901A15);国际科技合作计划项目(2003DFB00005;2004DFB00300)

摘　　要：运用mRNA差异显示技术(DDRT-PCR),并结合反向Northern杂交筛选麻疯树(Jatropha.curcus)干旱胁迫下差异表达基因,最终从麻疯树叶片中分离出8条干旱胁迫诱导下表达上调的差异片段.经克隆、测序和基因信息分析后发现其中4个片段与GenBank中已知序列有较高同源性.对这4个差异表达片段的序列分析表明:JcDD-3和JcDD-13分别与1-磷酸-肌醇合成酶(MIPS)基因及甜菜碱醛脱氢酶(BADH)基因序列有较高同源性;JcDD-16与白杨中度抗旱叶片中提取的表达序列标签(CU230383)核酸序列同源;JcDD-25与植物富含亮氨酸重复序列型类受体蛋白激酶(LRR-RLK)基因序列同源.mRNA Differential Display technology and reverse Northern blot were used to investigate the variation of resistance of related genes induced by drought stress in Jatropha curcus. 8 differential bands were observed up-regμLated. By cloning, sequencing and analysis of genetic information found four fragments share high similarity with that of the known sequences in GenBank. The results showed Jc- DD-3, JcDD-13, JcDD-16 and JeDD-25 share high similarity with that of 1-phosphate -inositol synthase （MIPS） gene , the betaine aldehyde dehydrogenase （BADH） gene, the expressed sequence tags （CU230383） which Cloned from the poplar leaves in moderate drought and leucine-rich repeat receptor protein kinase types （LRR-RLK） gene, respectively.

关 键 词：MRNA差异显示 干旱胁迫 麻疯树 

分 类 号：Q78[生物学—分子生物学]