牛奶尿素氮含量不同测定方法比较研究  

Comparison of Determination Methods for Milk Urea Nitrogen

作　　者：王东卫[1,2] 曹志军[1] 王之盛[2] 黄文明[1] 李胜利[1] 王瑜 温万 邵怀峰 

机构地区：[1]中国农业大学动物科学技术学院,北京100193 [2]四川农业大学动物营养研究所,雅安625014 [3]宁夏回族自治区畜牧工作站,银川750004

出　　处：《中国奶牛》2010年第9期55-57,共3页China Dairy Cattle

基　　金：公益性行业(奶业)科研专项经费(nyhyzx07-036);中国农业大学-南京农业大学青年教师开放基金(CN2007008)

摘　　要：本研究的目的是比较不同测定方法对牛奶尿素氮含量的影响。分别采用红外法(Foss 4000)、二乙酰-肟法及不同去蛋白处理的尿素氮试剂盒法测定奶样中尿素氮含量(n=50)。结果表明,红外法、二乙酰-肟法、尿素氮试剂盒水溶法的测定结果之间差异不显著(P>0.05)。试剂盒法中,三氯乙酸水溶液测定的牛奶尿素氮值显著高于乙醚溶解法的测定结果值(P<0.01)。因此,实验室中可以采用二乙酰-肟法和尿素氮试剂盒水溶法测定牛奶尿素氮含量。This objective of this study was to compare the difference of milk urea nitrogen（MUN）concentration determined by different measurement methods.Milk samples（n=50）were determined by infrared method,diacetyl monoxime method and urea nitrogen kit,respectively.The results showed that there was no significant difference in MUN concentration by the Fossomatic 4000 Milk Analyzer,diacetyl monoxime method and urea nitrogen kit（P〉0.05）.Compared with the distilled water solution with urea nitrogen kit,diethyl ether solution with urea nitrogen kit gave lower MUN concentration（P〈0.01）.The results indicated that the MUN concentration were similar between Fossomatic 4000 Milk Analyzer,diacetyl monoxime method and urea nitrogen kit.When MUN concentration was determinted by urea nitrogen kit,there was extremely significant difference between distilled water solution and diethyl ether solution.

关 键 词：牛奶尿素氮 红外法 二乙酰-肟法 尿素氮试剂盒法 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]