Characterization of expressed sequence tags obtained by SSH during root formation in Larix cuttings  

作　　者：FENG Jian QI Li-wang SUN Xiao-mei DONG Jian ZHANG Shou-gong 

机构地区：[1]Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, P. R. China [2]Liaoning Academy of Forestry Science, Shenyang 110032, P. R. China

出　　处：《Forestry Studies in China》2010年第3期137-141,共5页中国林学（英文版）

基　　金：supported by the National S&T Support Projects for the 11th Five-Year Plan (2006BA-D01A14);the National High-Tech "863" Program of China (2006AA100109);the National "973" Program (2009CB119107)

摘　　要：SSH was used to analyze gene transactivation during root formation of Larix cuttings. Two subtractive cDNA libraries were constructed from clone 31-6 as tester or driver and clone 15-4 as driver or tester. The SSH PCR products from the libraries were cloned into a pGEM-T easy vector and after PCR and dot blot analysis, positive clones were selected, sequenced and compared to the database in GenBank with BLASTX. The results of a sequence assembly in two libraries show that 521 UniEST （expressed sequence tag） were obtained. These 521 UniEST belong to metabolism, signal pathways, transport, resistance, developmental processes, local- ization, unknown proteins and ＂no hits found＂. All of these suggest that subtractive cDNA libraries during root formation of Larix cuttings were constructed successfully.SSH was used to analyze gene transactivation during root formation of Larix cuttings. Two subtractive cDNA libraries were constructed from clone 31-6 as tester or driver and clone 15-4 as driver or tester. The SSH PCR products from the libraries were cloned into a pGEM-T easy vector and after PCR and dot blot analysis, positive clones were selected, sequenced and compared to the database in GenBank with BLASTX. The results of a sequence assembly in two libraries show that 521 UniEST （expressed sequence tag） were obtained. These 521 UniEST belong to metabolism, signal pathways, transport, resistance, developmental processes, local- ization, unknown proteins and ＂no hits found＂. All of these suggest that subtractive cDNA libraries during root formation of Larix cuttings were constructed successfully.

关 键 词：suppression subtractive hybridization （SSH） Larix cuttings root formation 

分 类 号：Q78[生物学—分子生物学] S791.22[农业科学—林木遗传育种]