神经管缺陷大鼠模型胚胎形态学改变及PLA_2、COX-2表达  

Morphologic change and expression of PLA_2 and COX-2 in diabetes-induced embryopathy in rats

作　　者：沈瑾[1] 马向东[1] 招明高[2] 张卫华[2] 李杨[2] 

机构地区：[1]第四军医大学药学系,陕西西安710032 [2]第四军医大学西京医院妇产科,陕西西安710032

出　　处：《现代生物医学进展》2010年第17期3216-3220,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金(30200297)

摘　　要：目的:研究神经管缺陷大鼠模型胚胎形态学改变及PLA2、COX-2表达。方法:16只SD雌性大鼠分为2组:第一组为正常对照组,8只,受孕后正常饲养;第二组为糖尿病模型组,8只,受孕后第6天经过尾静脉注射链脲佐菌素(STZ)构建先天性神经管缺陷组大鼠。妊娠12日后取出所有胚胎做HE染色观察胚胎形态学及免疫荧光染色的方法观察PLA2、COX-2表达。结果:(1)HE染色及免疫荧光发现糖尿病胚胎的神经管形态发育明显畸形(2)免疫荧光发现PLA2、COX-2糖尿病胚胎的表达明显降低。结论:PLA2、COX-2基因表达在SD大鼠神经管的发育过程中起着重要的作用。可能对临床治疗神经管畸形提供了一种新方法。Objective：To detect the morphologic change and expression of PLA2 and COX-2in diabetes-induced embryopathy in rats.Method：Two groups of Sprague-Dawley rats were used.First group had 8 normal control rats with normal diet.Second group had 8 rats treated with streptozotocin（STZ）to induce diabetic with congenital neural tube defects in offspring;all yolk sac cells were harvested at day 12 of gestation from Two group.Embryo morphology HE staining and immunohistochemical experiments were performed to observe the PLA2 and COX-2 expression.Results：（1）HE staining and Immunofluorescence showed obvious morphological deformity differences in embryonic development of neural tube in the normal and diabetes rats.（2）Immunofluorescence showed embryonic yolk sac cell prostaglandin enzyme PLA2 and prostaglandin synthase COX-2 expression were decreased in experimental diabetic group.Conclusion：The expression of PLA2 and COX-2 genes was important in the development of neural tube in the SD rats.It could provide a novel method in the clinical therapy of neural tube defect.

关 键 词：神经管缺陷 PLA2 COX-2 糖尿病 动物模型 

分 类 号：Q954[生物学—动物学]