白藜芦醇浸提纯化过程在线检测条件的研究  

Study of on-Line Determination Conditions of Resveratrol During Extraction and Purification Resveratrol Process

作　　者：沈会明[1] 高丽娟[1] 刘依然[2] 姚腾豪[1] 张丽华[1] 王霞[1] 

机构地区：[1]辽宁科技大学化工学院 [2]辽宁省鞍山市第一中学

出　　处：《光谱实验室》2010年第5期1821-1823,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：辽宁科技大学第五届大学生科研训练项目资助

摘　　要：通过实验确定了虎杖白藜芦醇乙醇浸提液的HPLC检测条件。最佳检测条件为,Extend反相ODS柱0.46cm×15cm,紫外检测器检测波长240nm,V(甲醇)∶V(水)=50∶50的流动相,1mL/min的洗脱流速,室温。该方法灵敏、方便、可靠、快速,适用于白藜芦醇提取、纯化中的在线检测。Resveratrol was extracted from polygonum cuspidatum using ethanol as extraction solvent and determined by HPLC.The optimum detection conditions were：extend RP-ODS column 0.46cm×15cm,UV detector,240nm as detection wavelength,V（methanol）∶V（water）=50∶50 as mobile phase,elution flow rate of 1mL/min,room temperature.The method is sensitive,convenient,reliable and fast for on line detection of resveratrol and purification of resveratrol from polygonum cuspidatum.

关 键 词：虎杖 白藜芦醇 高效液相色谱 

分 类 号：O629[理学—有机化学] O657.72[理学—化学]