拉曼光谱技术在脂质过氧化反应中的应用  

Application of Raman Spectroscopy for Lipid Peroxidation

作　　者：沈金虎[1] 王亚利[2] 王俊玲[1] 王卫东[1] 秦广雍[1] 

机构地区：[1]河南省离子束生物工程重点实验室郑州大学物理工程学院,郑州市大学路75号450052 [2]郑州师范高等专科学校物理系,郑州市英才街6号450044

出　　处：《光谱实验室》2010年第5期1951-1955,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：农业部农业公益性行业科研专项经费项目"核技术农业应用"资助(200803034);国家自然科学基金项目资助课题(10505018)

摘　　要：本实验以亚油酸为材料,分别采用过氧化产物检测法和拉曼光谱技术对亚油酸的自氧化过程进行监测,从氧化反应的中间产物和底物的结构变化两个角度,对亚油酸的自氧化过程进行分析。为不饱和脂肪酸的过氧化机理研究提供实验证据。结果表明:拉曼光谱技术能够用来研究单分子物质的结构变化,随着自氧化过程的进行,亚油酸的拉曼光谱在谱型和峰强方面都有所变化,能够反映分子内部结构的变化。其时间效应与采用TBA和共轭二烯法测定时的时间效应基本一致。Taking linoleic acid as the material,linoleic acid oxidation process of self-monitoring was monitored by peroxidation product detecting method and Raman spectra.Self-oxidation process of linoleic acid was analyzed in two angles,the oxidation of production and substrate structural changes.It provides experimental evidence for peroxidation study of unsaturated fatty acid.The results showed that Raman spectroscopy can be used to study single-molecule substance structural changes.With the auto-oxidation process carrying out,the Raman spectra of linoleic acid changes both in spectral pattern and peak intensity.All these changes can reflect the changes in the structure of linoleic acid.Time effect detecting by Raman spectrum is mainly same as that using TBA and conjugated diene method measuring.

关 键 词：亚油酸 拉曼光谱 硫化巴比妥酸法 丙二醛 

分 类 号：O657.37[理学—分析化学]