双波长薄层扫描法测定刺山柑不同部位盐酸水苏碱的含量  

Determination of Stachydrine Hydrochloride in Different Parts of Capparis Spinona L. by Dual Wavelength TLC Scanning

作　　者：姜秀娟[1,2] 孟庆艳[1,2] 余明星[2] 白红进[1,2] 

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔市大学路843300 [2]塔里木大学生命科学学院,新疆阿拉尔市大学路843300

出　　处：《光谱实验室》2010年第5期1959-1963,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：新疆生产建设兵团科学技术局政策引导项目(2006YD29);塔里木盆地生物资源保护利用重点实验室开放项目(BR0609)

摘　　要：采用双波长薄层扫描法测定刺山柑不同部位盐酸水苏碱的含量。展开剂:正丁醇-盐酸-乙酸乙酯(8∶3∶1);波长分别为λS=525nm,λR=700nm。结果表明,盐酸水苏碱点样量在2.03—16.24μg范围内线性关系良好,平均回收率为96.83%,RSD=2.00%。刺山柑植株中盐酸水苏碱的含量从高到低依次为:根、花蕾、果实、茎及叶。该方法简便、准确、重现性好,可用于刺山柑不同部位盐酸水苏碱含量的测定。The content of stachydrine hydrochloride in different parts of Capparis spinona L.was determined by dual wavelength TLC scanning.Developer was n-butanol-hydrochloric acid-ethyl acetate（8∶3∶1）;wavelength was λS=525nm and λR=700nm respectively.The results showed that the average recovery was 96.83%,RSD was 2.00% in the linearity range of 2.03—16.24μg.The sequence of the content of stachydrine hydrochloride in different parts of Capparis spinona L.from the highest to the lowest was following：roots,buds,fruits,stems and leaves.The method is simple,accurate,reproducible.It can be used for the determination of the content of stachydrine hydrochloride in different parts of Capparis spinona L..

关 键 词：刺山柑 盐酸水苏碱 薄层扫描法 

分 类 号：R927.2[医药卫生—药学] O657.7[理学—分析化学]