Luminol-Cu(Ⅱ)体系流动注射化学发光法测定乙酰半胱氨酸  

Determination of Acetylcysteine in Luminol-Cu(Ⅱ) System by Flow Injection Chemiluminescence

作　　者：李红[1] 杨育民[1] 

机构地区：[1]渭南职业技术学院,陕西省渭南市杜化路4号714000

出　　处：《光谱实验室》2010年第5期1968-1970,共3页Chinese Journal of Spectroscopy Laboratory

摘　　要：发现了Luminol-Cu(Ⅱ)-乙酰半胱氨酸化学发光体系,探讨了影响化学发光反应的各个因素,建立了测定乙酰半胱氨酸的流动注射化学发光新方法。该方法的线性范围为7.0×10-10—1.0×10-7g/mL,检出限为2.0×10-11g/mL,相对标准偏差为1.0%(1.0×10-8g/mL,n=11)。该方法已用于药物制剂中乙酰半胱氨酸含量的测定。A new chemiluminescence system,Luminol-Cu（Ⅱ）-acetylcysteine was investigated.The experimental conditions that affected the chemiluminescence reaction were carefully optimized.Under the selected conditions,the concentration of acetylcysteine was linear related to the chemiluminescence intensity in the range of 7.0×10^-10—1.0×10^-7g/mL.The detection limit was 2.0×10^-11g/mL.The relative standard deviation was 1.0% for the 11 replicate determinations of 1.0×10^-8g/mL acetylcysteine solution.The method was applied to the determination of acetylcysteine in pharmaceutical preparations.

关 键 词：化学发光 流动注射 乙酰半胱氨酸 

分 类 号：O657.39[理学—分析化学]