麻疯树JcFAD7基因启动子克隆及植物表达载体构建  

Cloning of JcFAD7 promoter in Jatropha curcas L. and construction of plant expression vector

作　　者：曾玲[1,2] 吴平治[1,2] 魏倩[1,2] 姜华武[1] 吴国江[1] 李美茹[1] 

机构地区：[1]中国科学院华南植物园植物资源保护与可持续利用重点实验室,广东广州510650 [2]中国科学院研究生院,北京100049

出　　处：《广东农业科学》2010年第9期177-180,共4页Guangdong Agricultural Sciences

基　　金：中科院方向性项目(KSCX2-YW-G-035)

摘　　要：利用TAIL-PCR技术成功克隆了麻疯树JcFAD7基因起始密码子的上游1926bp序列。利用生物信息学手段对其序列特征和潜在的调控元件进行分析表明,该序列含有4个TATA-box和6个CAAT-box,还包含光响应元件、MYB结合位点、不同的激素反应元件以及各种胁迫响应元件,说明JcFAD7的表达可能受多种因素的调控。将启动子连接到pBI101.1载体,成功构建了驱动报告基因GUS的植物表达载体。为阐明麻疯树不饱和脂肪酸的合成代谢过程和植物生长发育的调控机理奠定了基础。The upstream sequence of start codon in JcFAD7 was cloned by using TAIL-PCR technology.The target fragment was connected with pMD18-T vector and the sequence length of 1 926 bp was obtained. In addition, the sequence characteristics and its potential regulatory elements were analyzed via bioinformatics means. There were four TATA-boxes and six CAAT-boxes in this sequence. What’s more, light responsive elements, MYB binding sites, various phytohormone responsive elements and stress responsive elements also were discovered in the promoter sequence. These suggested that the expression of JcFAD7 was regulated by multiple factors. Finally, the expression vector for driving reporter gene GUS was successfully constructed. This research had laid the foundation for clarifying the synthesis process of unsaturated fatty acids in Jatropha curcas and the regulatory mechanism of plant development.

关 键 词：麻疯树 ω-3脂肪酸脱氢酶 三烯脂肪酸 启动子 

分 类 号：Q785[生物学—分子生物学]