卡里佐枳橙幼苗病害致病菌的分离和鉴定  

Isolation and Identification of the Causal Agents of Citrange Seedling Diseases

作　　者：刘娟[1,2] 胡军华[2] 姚廷山[2] 李鸿筠[2] 冉春[2] 刘浩强[2] 徐丽兰[1,2] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]中国农业科学院柑桔研究所

出　　处：《中国南方果树》2010年第5期4-7,共4页South China Fruits

摘　　要：在卡里佐枳橙幼苗上发生一种严重病害,为害卡里佐枳橙茎干,造成褐变,叶片干枯或脱落,严重时植株死亡。从卡里佐枳橙病害苗上分离纯化得到4株真菌,分别为MP1、MP2、MP3和MP4。致病性测定发现,上述4种真菌的致病程度不同,MP2孢子悬浮液处理发病最轻,MP3孢子悬浮液处理发病最为严重;对这4种菌进行形态学和分子生物学鉴定,发现3株属于链格孢属(Alternariasp.),另外1株属于盾壳霉属(ConiothyriumCorda)。A serious citrange seedling disease that caused stem browning and leaf dehydration and abscission,and even death of the whole tree was studied.Four fungi,MP1、MP2、MP3 and MP4,were isolated from diseased seedlings.Inoculation test showed that these strains varied in their pathogenicity,and the least pathogenic isolate was MP2 while the most pathogenic isolate was MP3.Morphological and molecular studies identified that 3 of the isolates belong to Alternaria and the rest belongs to Coniothyrium Corda after identified by methods of morphology and molecular biology.

关 键 词：卡里佐枳橙幼苗致病菌 链格孢 致病性 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]