肝乐颗粒抗四氯化碳诱导的大鼠肝纤维化实验研究  

Experimental Study on Ganle Granule in Rats Counteracting Hepatic Fibrosis Induced by Carbon Tetrachloride

作　　者：姜辉[1] 吴芙蓉[2] 夏伦祝[1] 张睿[1] 

机构地区：[1]安徽中医学院第一附属医院药学部,合肥230031 [2]安徽医科大学药学院,合肥230032

出　　处：《中药新药与临床药理》2010年第5期480-483,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：安徽中医学院临床科研基金(2008lcky011)

摘　　要：目的探讨肝乐颗粒抗四氯化碳诱导的大鼠肝纤维化作用及其可能的作用机制。方法除正常组外,其余各组采用四氯化碳诱导肝纤维化模型,每周2次,连续12周。于造模第7周起,给药组分别灌胃给予相应的受试药物,正常组和模型组灌胃给予等容量生理盐水,疗程6周。实验结束后,分光光度法测定MDA、SOD、NOS和iNOS的含量,ELISA法测定血清TIMP-1水平;同时取固定部位肝脏组织,免疫组化技术测定肝组织中TIMP-1蛋白的表达,RT-PCR技术测定肝组织中TIMP-1mRNA的表达。结果肝乐颗粒各剂量组能明显降低肝纤维化大鼠MDA、NOS、iNOS、TIMP-1的含量,升高SOD水平,抑制肝纤维化大鼠肝组织中TIMP-1蛋白和TIMP-1mRNA的表达。结论肝乐颗粒有一定的抗肝纤维化作用,抑制肝纤维化大鼠TIMP-1的表达可能是其抗纤维化的作用机制之一。Objective To investigate the inhibitory effect and possible mechanism of Ganle Granule（GG）on hepatic fibrosis induced by carbon tetrachloride（CCl4）in rats.Methods Rats hepatic fibrosis was induced by CCl4 twice a week for 12 weeks.Since the 7th week,GL was given to model rats daily by intragastric administration for 6 weeks.Contents of malondialdehyde（MDA）,superoxide dismutase（SOD）,nitric oxide synthase（NOS）and iNOS were detected by absorption spectrometry.The serum TIMP-1 level was examined by enzyme-linked immunospecific assay（ELISA）.Hepatic tissue in certain position was sampled,and TIMP-1 expression in the hepatic tissue was detected by immunohistochemical method.Moreover,TIMP-1mRNA expression in liver tissue was detected by RT-PCR technology.Results GG could significantly decrease MDA,NOS,iNOS and TIMP-1 contents,increase SOD level,and inhibit the protein and mRNA expression of TIMP-1 in hepatic tissue of hepatic fibrosis rats.Conclusion GL has an obvious anti-hepatic fibrosis action,and effectively inhibiting the expression of TIMP-1 may be one of its therapeutic mechanisms.

关 键 词：肝乐颗粒 肝纤维化 基质金属蛋白酶抑制剂-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]