反相高效液相色谱法测定狼毒中岩大戟内酯B的含量  

RP-HPLC Determination of Jolkinolide B in Euphorbiae ebracteolatae

作　　者：王灿坚[1,2] 严小红[2] 谢演晖[2] 江英桥[2] 刘东辉[1] 马双成[3] 

机构地区：[1]广州中医药大学,广州510405 [2]广州市药品检验所,广州510160 [3]中国药品生物制品检定所,北京100050

出　　处：《中药新药与临床药理》2010年第5期521-524,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技支撑计划药品安全关键技术研究(2006BAI14B00)资助课题"常见与重要药品安全标准研究"(2006BAI14B01)

摘　　要：目的建立狼毒药材中岩大戟内酯B的含量测定方法。方法狼毒药材经粉碎后利用乙醇超声提取,采用高效液相色谱法对岩大戟内酯B的含量进行测定,色谱条件如下:C18柱(250mm×4.6mm,5μm),流动相:乙腈-水(65:35),流速:1.0mL/min,检测波长为238nm,进样量10μL,外标法定量。结果岩大戟内酯B在4.070～32.560μg/mL范围内线性关系良好,r=0.9999,平均加样回收率为98.02%,RSD为1.02%。结论该方法专属性强,快速简便,可作为狼毒药材中岩大戟内酯B的含量测定方法,可为狼毒质量标准的制定提供相关依据。Objective To establish a method for the determination of jolkinolide B in Euphorbiae ebracteolatae.Methods The medicinal material of Euphorbiae ebracteolatae.was extracted with ethanol after comminution.RPHPLC was applied.A C18 column（250 mm×4.6 mm,5 μm） was used with Acetonitrile-Water（65：35）as the mobile phase,the flow rate was 1.0 mL/min with an injection volume of 10μL,and the detection wavelength was at 238 nm.Results The calibration curve was linear in the range of 4.070-32.56 μg/mL of jolkinolide B with correlation coefficient of 0.9999.The average recovery was 98.02 % with RSD being 1.02 %.Conclusion The method is simple,rapid and reliable,which can be regarded as a reasonable method for determination of jolkinolide B in Euphorbiae ebracteolatae.The results will supply evidence for developing quality specification of Euphorbiae ebracteolatae.

关 键 词：狼毒 岩大戟内酯B 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]