紫花苜蓿高频组培再生体系影响因素研究  

Study on factors affecting high frequency tissue culture of alfalfa regeneration system

作　　者：徐春波[1] 王勇[2] 赵海霞[1] 李兴酉[1] 

机构地区：[1]中国农业科学院草原研究所,内蒙古呼和浩特010010 [2]内蒙古农业大学生态环境学院,内蒙古呼和浩特010018

出　　处：《草原与草坪》2010年第4期55-58,63,共5页Grassland and Turf

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)项目资助(2006-1-01)

摘　　要：用紫花苜蓿4个品种获得高频再生体系,研究了基因型、外植体、培养基对紫花苜蓿脱分化和分化的影响。结果表明,WL-323的愈伤组织分化率最高达到76.3%,为最佳基因型;下胚轴是最佳的外植体;最佳脱分化培养基为改良SH+4.0 mg/L 2,4-D+0.5 mg/L 6-BA,最佳继代培养基为MSO+0.5 mg/L NAA+1.0 mg/L 6-BA+1.0 mg/L AgNO3,最佳分化培养基为MS。The high frequent regeneration system of 4 alfalfa cultivars were obtained and the effects of genotype,explants and medium on dedifferentiation and differentiation were studied.The results showed that the callus differentiation rate of WL-323 was 76.3%,it is the optional genotype.The best optimal explants was hypocotyls.The optimal dedifferentiation medium was SH＋4.0 mg/L 2,4-D＋0.5⒐mg/L 6-BA.The optional subculture medium was MSO＋0.5 mg/L NAA＋1.0 mg/L 6-BA＋1.0 mg/L AgNO3.The optimal differentiation medium was MS.

关 键 词：紫花苜蓿 基因型 外植体 培养基 脱分化 分化 

分 类 号：S54[农业科学—作物学] S335