山西地区褐土胶质芽孢杆菌生物学特性研究  

Study on biological characteristics of Bacillus mucilaginosus isolated from cinnamon soil in Shanxi

作　　者：张晓波[1] 赵艳[2] 樊俊华[1] 

机构地区：[1]海南大学农学院,海南儋州571737 [2]山西农业大学资源环境学院,山西太谷030801

出　　处：《草原与草坪》2010年第4期89-92,96,共5页Grassland and Turf

基　　金：海南省自然科学基金(309009)"结缕草根际联合固氮菌的分离;鉴定及筛选";海南省教育厅高等学校科研项目(Hjkj2010-16)"海南省不同暖季型草坪草根际胶质芽孢杆菌遗传多样性及解钾活性的研究"资助

摘　　要：从山西地区褐土中筛选出6株胶质芽孢杆菌,以辽宁菌种保藏中心提供的胶质芽孢杆菌（编号K0）为参照菌株,对其形态学、生理生化特性、耐盐性、耐酸碱性、温度敏感性等特性进行了测定。结果表明,这些菌株均为革兰氏阴性杆菌,产生圆形芽孢和丰厚的荚膜,菌落透明隆起,粘性强;过氧化氢酶试验呈阳性,能水解淀粉,VP阴性,不产生吲跺。K5、K0在3%NaCl浓度的培养基上能生长;在25~30℃供试胶质芽孢杆菌均能良好生长。研究可为高效胶质芽孢杆菌菌株材料的获得以及研制生物钾肥提供实践及理论基础。Six strains of Bacillus mucilaginosus were screened from cinnamon soil in Shanxi,and the physiological and biochemical characters,salt tolerance,acid and alkali resistance,and temperature sensitivity were determined by using the modal strain（strain code：K0） from Liaoning Center for Microorganism Reserve.The results showed that all strains were gram negative bacteria with circular gemma and thick capsule;the catalase test was masculine.They could hydrolyze starch and the V.P test was feminine.Strains K5 and K0 could survive in the medium with 3% NaCl and grow well under the temperature ranging from 25 ℃ to 30 ℃.The result could provide basis to obtain efficient strains for bio-fertilizer development.

关 键 词：胶质芽孢杆菌 褐土 生物学特性 

分 类 号：S143.3[农业科学—肥料学]