Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)  

作　　者：CHEN Tao TIAN Meng-xiang ZHANG Ya-dong ZHU Zhen ZHAO Ling ZHAO Qing-yong LIN Jing ZHOU Li-hui WANG Cai-lin 

机构地区：[1]Jiangsu High Quality Rice Research and Development Center, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences,Nanjing 210014, China,＂ #These authors contributed equally to this paper

出　　处：《Rice science》2010年第3期173-178,共6页水稻科学（英文版）

基　　金：supported by the National Transgenic Crops Program, China (Grant No. 2008ZX08001-006);the Research Funds for Public Benefit in Ministry of Agriculture, China (Grant No. 200803056);the Key Support Program of Jiangsu Science and Technology, China (Grant No. BE2008354);the Self-directed Innovation Fund of Agricultural Science and Technology in Jiangsu Province, China (Grant No. CX [08]603)

摘　　要：Rice with low glutelin content is suitable as functional food for patients affected by kidney failure. Low glutelincontent gene Lgc1 in rice has a 3.5-kb deletion between two highly similar glutelin genes GluB4 and GluB5, which locates on the short arm of chromosome 2. To improve the selection efficiency in low glutelin-content rice breeding, two molecular markers designated as InDel-Lgc1-1 and InDel-Lgc1-2 were developed to detect the low glutelin-content gene Lgc1. A double PCR detection indicated that combined use of the two markers could easily distinguish the genotypes of Lgc1 from different rice varieties. Therefore, as a simple and low-cost technique, the molecular marker could be widely used to identify different varieties with Lgc1 gene and applied in marker-assisted selection of low glutelin-content rice.

关 键 词：Oryza sativa low glutelin-content gene functional marker double polymerase chain reaction 

分 类 号：S852.65[农业科学—基础兽医学] S511[农业科学—兽医学]