Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene  

作　　者：CHEN De-xi CHEN Xue-wei LEI Cai-lin MA Bing-tian WANG Yu-ping LI Shi-gui 

机构地区：[1]Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China [2]Rice Research Institute, SichuanAgricultural University, Chengdu 611130, China [3]Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing100081, China

出　　处：《Rice science》2010年第3期179-184,共6页水稻科学（英文版）

基　　金：supported by the Program for Supporting New-century Excellent Talents of Ministry of Education, China (Grant No. NCET-04-0907);the Program for Supporting Development of Innovative Research Teams of Ministry of Education, China (Grant No. IRT0453);the Applied Basic Research Program of Sichuan Province, China (Grant No. 2008JY0023-1)

摘　　要：Resistance to rice blast of transgenic rice lines harboring rice blast resistance gene Pi-d2 transformed from three different expression vectors of pCB6.3kb, pCB5.3kb and pZH01-2.72kb were analyzed. Nine advanced-generation transgenic rice lines with Pi-d2 gene displayed various resistance to 39 rice blast strains, and the highest disease-resistant frequency reached 91.7%. Four early-generation homozygous transgenic lines with Pi-d2 gene exhibited resistance to more than 81.5% of 58 rice blast strains, showing the characteristic of wide-spectrum resistance. The transgenic embryonic calli selected by the crude toxin of rice blast fungus showed that the callus induction rate of immature embryo from transgenic rice plants decreased as the concentration of crude toxin in the culture medium increased. When the concentration of crude toxin reached 40%, the callus induction rate of immature embryo from transgenic lines was 49.3%, and that of the receptor control was 5%. The disease incidence of neck blast of the transgenic rice lines in fields under induction was 0% to 50%, indicating that the rice blast resistance of transgenic rice lines is much higher than that of the receptor control.

关 键 词：transgenic rice disease resistance gene rice blast resistant spectrum crude toxin callus induction 

分 类 号：S511.01[农业科学—作物学] S435.111.4