乙型肝炎患者PreS1Ag、HBV-DNA以及肝功能联合检测分析  

Combined detection of Hepatitis B PreS1Ag,HBV-DNA and liver function

作　　者：易伟莲[1] 崔国辉[1] 欧超伟[1] 袁汉尧[1] 

机构地区：[1]广东医学院附属医院检验科,广东湛江524001

出　　处：《中国医药导报》2010年第27期71-72,共2页China Medical Herald

摘　　要：目的:通过对乙肝患者进行"乙肝两对半"、前S1抗原(PreS1Ag)、HBV-DNA以及肝功能的联合检测,了解乙肝患者各项指标的相关性以及HBV-DNA和PreS1Ag阳性患者及乙肝五项不同阳性组别与肝功能的损害关系。方法:"乙肝两对半"、前S1抗原采用酶联免疫吸附试验(ELISA)检测,HBV-DNA采用荧光定量PCR法检测,肝功能采用日本奥林巴斯AL2700全自动生化分析仪检测。结果:215例乙肝五项不同阳性组别的患者中共有189例前S1抗原阳性,阳性率为87.91%,其中有109例HBeAg阳性患者前S1抗原阳性为102例,HBV-DNA阳性为98例,符合率为96.08%(98/102),差异无统计学意义(P>0.05)。109例"大三阳"患者谷丙转氨酶(ALT)升高者占68.81%,63例"小三阳"患者ALT升者占38.10%,两者差异有统计学意义(P<0.05)。结论:PreS1Ag不仅能够反映乙肝病毒的复制情况,而且更是病毒感染性的指标,与HBeAg、HBV-DNA、ALT呈正相关,这对乙肝患者的诊治和疗效具有很好的临床应用价值。Objective：To detect ＂two pairs of semi-hepatitis B＂,the former S1 antigen（PreS1Ag）,HBV-DNA and liver function of hepatitis B patients,in order to understand the relevance of the indicators of hepatitis B patients,as well as the relationship between liver function damage and PreS1Ag and five different groups of hepatitis B.Methods：＂Two pairs of semi-hepatitis B＂ and the former S1 antigen were detected by ELISA,HBV-DNA by fluorescence quantitative PCR and liver function by Olympus AL2700 automatic biochemical analyzer.Results：In 215 cases positive patients of five different groups of hepatitis B,there were 189 cases positive patients of the former S1 antigen,the positive rate was 87.91%.There were 109 cases HBeAg positive patients who included 102 cases positive patients of the former S1 antigen and 98 cases positive patients of the HBV-DNA,the coincidence rate was 96.08%（98/102）,the both difference had no statistical significance（P0.05）.But ALT elevation rate was 68.81% in 109 cases ＂big three positive＂ patients,it was 38.10% in 63 cases ＂small three positive＂ patients,the both difference had statistical significance（P0.05）.Conclusion：PreS1Ag not only reflects the hepatitis B virus replication,but it is the viral infectious target and related with HBeAg,HBV-DNA,ALT.It has good clinical practice value to diagnosis and curative effect of the hepatitis B patients.

关 键 词：乙型肝炎 PRES1AG HBV-DNA 肝功能 

分 类 号：R446[医药卫生—诊断学] R512.62[医药卫生—临床医学]