AMOS-PCR在宁夏布鲁氏菌种型鉴定中的应用  

Use of AMOS-PCR to identify species of Brucella in Ningxia

作　　者：马学平[1] 高建炜[1] 秦迎旭[1] 宋晓佳[1] 

机构地区：[1]宁夏疾病预防控制中心,宁夏银川750004

出　　处：《中国病原生物学杂志》2010年第9期652-654,共3页Journal of Pathogen Biology

基　　金：宁夏自然科学基金项目(No.NZ10201)

摘　　要：目的评价AMOS-PCR方法在布鲁氏菌种型鉴定中的实用性,为快速鉴定布鲁氏菌提供辅助方法。方法血培养法分离布鲁氏菌,用常规方法和AMOS-PCR对牛种104M疫苗株、羊种M5株和宁夏分离的5株布鲁氏菌进行种型鉴定。结果 5个分离菌株经AMOS-PCR均扩增出731和178 bp的特异性条带,鉴定为羊种布鲁氏菌。结论 AMOS-PCR在布鲁氏菌鉴定中具有特异、快速、准确等特点,适合作为一种辅助鉴定方法推广应用。Objective To evaluate the practicality of using AMOS-PCR to identify species of Brucella in order to provide a rapid method of identifying species of Brucella.Methods To isolate Brucella bacteria by blood culture and identify B.abortus 104M,B.melitensis M5,and five Brucella spp.from Ningxia using conventional methods and AMOS-PCR.Results Specific fragments of the five Bacteria spp.from Ningxia were identified by AMOS-PCR as B.melitensis.Conclusion An AMOS-PCR assay appears to be a rapid,simple,and specific method of identifying Brucella spp.

关 键 词：AMOS-PCR 布鲁氏菌 种型鉴定 宁夏 中国 

分 类 号：R378.52[医药卫生—病原生物学]