白花蛇舌草提取物对结肠癌HT-29细胞bcl-2和bax表达的影响  

Effect of Hedyotic Diffusa Extract on Expression of Bcl-2 and Bax in Human Colon Cancer HT-29 Cells

作　　者：彭军[1] 林久茂[1] 魏丽慧[1] 徐伟[2] 

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350108 [2]福建中医药大学药学院,福建福州350108

出　　处：《福建中医学院学报》2010年第5期23-26,共4页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省自然科学基金资助课题(2010J01195);福建省中西医结合老年性疾病重点实验室开放课题(2008J1004)

摘　　要：目的观察白花蛇舌草提取物在体外对结肠癌HT-29细胞株增殖和bcl-2和bax表达的影响。方法采用人结肠癌细胞HT-29细胞常规体外培养,随机设空白组和不同浓度白花蛇舌草提取物组,干预24h,倒置显微镜观察细胞形态变化;MTT法测定不同浓度药物对HT-29细胞增殖的影响;RT-PCR检测药物作用后的bax和bcl-2的表达。结果白花蛇舌草提取物干预后,HT-29的细胞密度明显减少,细胞变小;抑制HT-29细胞增殖,并呈现出量-效作用;bax的表达随药物剂量增加而增加,bcl-2的表达随药物剂量增加而减少。结论白花蛇舌草提取物能显著抑制人结肠癌HT-29细胞的增殖,通过上调bax和下调bcl-2的表达来诱导细胞凋亡,起到抗HT-29细胞的作用。Objective To investigate the effect of Hedyotic diffusa extract on the proliferation of human colon cancer HT-29 cells and expression of Bcl-2 and Bax in vitro. Methods The human colon cancer HT-29 cells were cultured in vitro and intervened by Hedyotic diffusa extract at different concentrations. After 24 hours, morphologic change of cells was observed under phase contrast microscope, cell viability was determined by MTT assay, and mRNA and protein expression levels of Bcl-2 and Bax were measured by RT-PCR and Western Blot. Results The density of HT-29 cells decreased obviously, the cells became smaller, and the proliferation of HT-29 cells was inhibited by Hedyotic diffusa extract in dose dependent manner. The expression of Bax increased with the increase of the drug concentration, and the expression of Bel-2 decreased with the increase of the drug concentration. Conclusion Hedyotic diffusa extract can significantly inhibit the proliferation of HT-29 cells, and induce ceils apoptosis by up-regulating the expression of Bax and down-regulating the expression of Bcl-2.

关 键 词：白花蛇舌草 结肠癌 HT-29细胞 BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学]