HPLC测定止嗽定喘丸中苦杏仁苷的含量  

HPLC determination of amygdalin content in Zhikedingchuan Pellet

作　　者：魏双元[1] 税丕先[2] 

机构地区：[1]四川省绵阳市中医院,四川绵阳621000 [2]四川省泸州医学院药学院,四川泸州646000

出　　处：《中国民族民间医药》2010年第19期39-40,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立止嗽定喘丸中苦杏仁苷的含量测定方法。方法:采用高效液相色谱法,流动相为水-甲醇-乙腈(75:20:5),检测波长为210nm。结果:止嗽定喘丸中苦杏仁苷平均回收率为99.016%,RSD为1.000%,含量为0.5233mg.g-1,即0.0313mg.丸-1。结论:该法操作简便、准确,可作为止嗽定喘丸中苦杏仁苷的含量控制方法。Objective：High-performance liquid chromatography was employed for determination of amygdalin content in Zhikedingchuan Pellet.Methods：HPLC was adopted and the mobile phase was water-methanol-acetonitrile（70：25：5）.The detective wavelength was 210nm.Results：The average recovery of amygdalin in Zhikedingchuan Tablet was 99.016% with RSD of 1.000% and the content was 0.5233mg/g（0.0313mg/P）.Conclusion：Besides simplicity,the method yields accurate reproducible results and can therefore be used in the quality control of amygdalin content in Zhikedingchuan Pellet.

关 键 词：止嗽定喘丸 高效液相色谱 苦杏仁苷 

分 类 号：R927.2[医药卫生—药学]