Effects of phosphorus and nitrogen limitation on PHA production in activated sludge  

作　　者：Qinxue Wen,Zhiqiang Chen,Ting Tian,Wei Chen State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China 

出　　处：《Journal of Environmental Sciences》2010年第10期1602-1607,共6页环境科学学报（英文版）

基　　金：support of the National Natural Science Foundation of China(No. 50807025);the China Postdoctoral Science Foundation (No.20080430921);the Science Foundation of Harbin (No.2007RFLXS013);the State Key Lab of Urban Water Resource and Environment(No.08UWQA06)

摘　　要：The effects of phosphorus and nitrogen limitation on polyhydroxyalkanoate （PHA） production and accumulation by activated sludge biomass with acetate as a carbon source were investigated. Pre-selected influent carbon-phosphorus （C：P, W/W） of 100, 160, 250, 500 and 750, and carbon-nitrogen （C：N, W/W） of 20, 60, 100, 125 and 180 were applied in the phosphorus limitation experiments and the nitrogen limitation experiments, respectively. The maximum PHA accumulation up to 59% of the cell dry weight with a PHA productivity of 1.61 mg PHA/mg COD consumed was observed at the C：N 125 in the nitrogen limitation experiment. This value was much higher than that obtained in previous studies with a normal substrate feeding. The study showed that activated sludge biomass would produce more polyhydroxybutyrate than polyhydroxyvalerate under the stress of nutrient limitation, especially under phosphorus limitation conditions. The experimental result also indicated that both phosphorus and nitrogen limitation may cause sludge bulking.The effects of phosphorus and nitrogen limitation on polyhydroxyalkanoate （PHA） production and accumulation by activated sludge biomass with acetate as a carbon source were investigated. Pre-selected influent carbon-phosphorus （C：P, W/W） of 100, 160, 250, 500 and 750, and carbon-nitrogen （C：N, W/W） of 20, 60, 100, 125 and 180 were applied in the phosphorus limitation experiments and the nitrogen limitation experiments, respectively. The maximum PHA accumulation up to 59% of the cell dry weight with a PHA productivity of 1.61 mg PHA/mg COD consumed was observed at the C：N 125 in the nitrogen limitation experiment. This value was much higher than that obtained in previous studies with a normal substrate feeding. The study showed that activated sludge biomass would produce more polyhydroxybutyrate than polyhydroxyvalerate under the stress of nutrient limitation, especially under phosphorus limitation conditions. The experimental result also indicated that both phosphorus and nitrogen limitation may cause sludge bulking.

关 键 词：POLYHYDROXYALKANOATE ACETATE nitrogen limitation phosphorus limitation sludge bulking 

分 类 号：X703[环境科学与工程—环境工程] F014.1[经济管理—政治经济学]