铂化微酶电极低极化电位下催化H_2O_2的能力  

0Enhance the Sensitivity of Oxidation of H 2O 2 on Platinized Platinum Wire at Low Working Potential

作　　者：刘百祥[1,2] 李青山 张兆奎[1,2] 

机构地区：[1]华东理工大学物理系 [2]华东理工大学生物反应器工程国家重点实验室

出　　处：《华东理工大学学报（自然科学版）》1999年第2期194-197,共4页Journal of East China University of Science and Technology

基　　金：国家自然科学基金

摘　　要：铂丝电极经铂化处理后，将３位取代的吡咯衍生物（３－羰基丁酸吡咯）与单体吡咯以适当的比例混合，与葡萄糖氧化酶（ＧＯＤ）一起用循环伏安法共聚合到铂化的铂丝电极上，形成杂聚吡咯／ＧＯＤ膜，制成微葡萄糖酶电极。酶电极在低的极化电位下对Ｈ２Ｏ２有较高的灵敏度，电极的稳定性良好，间断测定３个月，灵敏度仍达原来的７０％左右。Glucose oxidase was entraped onto a platinized platinum wire (0.8cm length,  300μm) by a heteropolypyrrole film, which was formed by electropolymerizing a mixture of glucose oxidase, pyrrole and a substituted pyrrole, 4 (3 pyrrolyl) 4 oxobutyric acid. The micro glucose oxidase electrode showed high sensitivity to H 2O 2 at low potential and could signifinicantly reduce the response to electroactive compounds such as ascorbic acid, uric acid and 4 acetamidophenol, after 3 months examination, only 30% of its originial sensitivity was lost.

关 键 词：铂化 酶电极 葡萄糖 杂聚吡咯膜 生物传感器 

分 类 号：Q532.303[生物学—生物化学] Q－331