禽流感HA基因疫苗脂质体的制备及其理化性质  

Preparation and Physicochemical Properties of Recombinant AIV HA Gene Liposome

作　　者：陈吉祥[1] 李广林[1] 薛飞群[1] 陈化兰 于康震[1] 赵荣材[1] 李树本[2,3] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所 [2]中国科学院兰州化学物理研究所 [3]中国农业科学院哈尔滨兽医研究所

出　　处：《中国兽医学报》1999年第3期230-232,共3页Chinese Journal of Veterinary Science

基　　金：国家"九五"攻关项目

摘　　要：用改良的逆相蒸发法制备了Ｈ７亚型禽流感保护性抗原血凝素（ＨＡ）基因重组质粒ｐＳＶＨ７脂质体。电镜观察表明，该脂质体多呈球形的单层或多层结构，脂质体粒径分布均匀，粒径范围２０～７００ｎｍ，平均粒径１６３ｎｍ，主要粒径分布位于５０～２００ｎｍ（８４．８０％）。重组质粒ＤＮＡ在脂质体的制备过程中保持了稳定性，并能抵抗ＤＮＡ酶的水解破坏作用。吸收光谱表明，在２４８ｎｍ及２６８ｎｍ处脂质体有２个特征吸收峰，ＤＮＡ的存在不改变吸收峰的位置，但能增加吸收峰的强度。AIV HA gene liposomes were prepared by a modified reverse phase evaporation method and the physicochemical properties were studied. Electron microscope observations showed that the liposome suspension was heterogeneous and the particle size ranged from 20 nm to 700 nm with a average diameter of 163 nm. The vesicles showed unilamellar and multilamellar structure which consisted of several concentric bilayers. The plasmid DNA remained intact and could be stored at 4 for at least one month in liposomes, it was also resistent to DNase I digestion. The liposome suspension had two absorption peaks at 248 nm and 268 nm on the ultraviolet spectra, addition of plasimd DNA increased absorption of the liposomes but did not change the position of absorption peaks.

关 键 词：禽流感病毒 血凝素基因 疫苗 脂质体 理化性质 

分 类 号：S858.326[农业科学—临床兽医学] Q78[农业科学—兽医学]