伊氏锥虫动基体DNA序列比较  

Comparison of Kinetoplast DNA Sequences of Trypanosoma evansi Strains in China

作　　者：王云飞[1] 周金林[1] 周勇志[1] 钟淑梅[1] 沈杰[1] 

机构地区：[1]上海家畜寄生虫病研究所

出　　处：《中国兽医学报》1999年第3期270-272,共3页Chinese Journal of Veterinary Science

摘　　要：对伊氏锥虫新疆骆驼株（ＸＪＣＡ）、安徽水牛株（ＡＨＢ）和云南水牛株（ＹＮＢ）的动基体ＤＮＡ进行ＰＣＲ扩增后，用Ｓａｎｇｅｒ双脱氧核糖核酸法测定扩增产物的序列。结果表明，各虫株间ＤＮＡ序列同源性为９７％。内聚分析表明，ＡＨＢ株与ＸＪＣＡ株相似性高属一类，而ＹＮＢ株与中国伊氏锥虫ＳＨ株相似性高属另一类。证明我国不同伊氏锥虫在动基ＤＮＡ序列上存在一些差别，这可能由于点突变的缘故。The kinetoplast DNA of Trypanosoma evansi isolates, XJCA strain, AHB strain and YNB strain, were amplified by PCR, then the DNA sequences of the PCR products were analysed by the dideoxy chain termination method of Sanger. The results showed that the homogeneity among the different strains was 97%. The clustering analysis showed that AHB strain and XJCA strain formed a group, while YNB strain and SH strain formed another group. It was proved that minor difference existed in KDNA sequences among variant Trypanosoma evansi strains in China, as could be explained by point mutant.

关 键 词：伊氏锥虫 动基体 DNA 序列分析 PCR 

分 类 号：S852.72[农业科学—基础兽医学]