应用酶标SPA Dot-ELISA同时检测牛羊血吸虫病和肝片吸虫病  

Simultaneous Diagnosis of Schistosomiasis and Fascioliasis in Cattle and Sheep by Enzyme Labelled SPA DotELISA

作　　者：张雪娟[1,2,3] 孙仁寅[1,2,3] 冯尚连 刘恩勇[1,2,3] 陈文龙 黄熙照[1,2,3] 杨继宗 

机构地区：[1]浙江省农业科学院畜牧兽医研究所 [2]湖北省畜牧局 [3]浙江省兰溪市农业局

出　　处：《中国兽医学报》1999年第3期273-274,共2页Chinese Journal of Veterinary Science

摘　　要：采用给雄性家兔注射黄牛、水牛、山羊ＩｇＧ混合液（１∶１∶１）制备的兔抗黄牛、水牛和山羊ＩｇＧ连接酶标ＳＰＡ，解决了酶标ＳＰＡ与牛羊血吸虫和肝片吸虫免疫复合物相结合的技术关键，并制成了可同时用于牛羊血吸虫病和肝片吸虫病诊断的酶试剂。建立了Ｄｏｔ－ＥＬＩＳＡ同时检测牛羊血吸虫病和肝片吸虫病技术，能测出人工感染１～１５０条虫体７～４２ｄ病畜抗体，阳性检出率达１００％。A key technique on combining enzyme labelled SPA with immunological complex of Schistosoma and Fasciola hepatica in cattle and sheep was solved by means of enzyme labelled SPA joined to rabbit antiIgG of yellow cattle buffalo and goat. An enzyme agent for simultaneous diagnosis of schistosomiasis and fascioliasis in cattle and sheep was formulated. The developed technique on simultaneous diagnosis of schistosomiasis and fascioliasis in cattle and sheep by DotELISA is able to detected the antibodies from the animals artificially infected with 1150 Schistosoma or F.hepatica 742 days post infection with the positive rate for both Schistosoma and F.hepatica being at 100%.

关 键 词：酶标SPA DOT-ELISA 血吸虫病 肝片吸虫病 牛 羊 

分 类 号：S858.235[农业科学—临床兽医学] S858.260.5[农业科学—兽医学]