一株聚β-羟基丁酸(PHB)产生菌的分离纯化及鉴定  

Isolation and identification of a strain of bacteria producing PHB

作　　者：李晓军[1,2] 赵旭[3] 常思静[3] 景春娥[3] 薛林贵[3] 

机构地区：[1]西北师范大学,甘肃兰州730070 [2]甘肃省高等学校招生办公室 [3]兰州交通大学

出　　处：《中兽医医药杂志》2010年第5期40-43,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：铁道部科技研究开发计划No:2010Z001E;甘肃省科技支撑计划(No:D90NKCA079);国家自然基金(No:3087038)

摘　　要：本研究从土样中分离纯化出一株PHB高产菌株P-9,通过对其进行形态学和生理生化反应特征研究,初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis)。进一步研究P-9菌的生长规律,发现P-9菌生长的延迟期为0-24 h,24 h后进入对数生长期,同时积累PHB,84 h时细胞进入稳定生长期,108 h时PHB产量达到最大,达1.25 g/L。A strain of P-9 bacteria with high produce of PHB was isolated and purified from soil.P-9 was preliminary appraised as a strain of Bacillus thuringiensis by its characteristics of form and physiology and biochemistry reactions.The lag phase,logarithmic phase and stationary phase was 0-24 h,24-84 h and 84 h,respectively.Further research showed that the maximum yield of PHB in P-9 was 1.25 g/L at 108 h.

关 键 词：聚Β-羟基丁酸 细菌 分离 纯化 

分 类 号：S859.7[农业科学—临床兽医学]