高速逆流色谱分离独活中水合氧化前胡素  

Separation of Oxypeucedanin Hydrate from Radix Angelicae Pubescentis by High-Speed Counter-Current Chromatography

作　　者：刘佳川[1] 于丽丽[1] 艾萍[2] 袁黎明[2] 

机构地区：[1]辽宁医学院化学教研室,锦州121001 [2]云南师范大学化学化工学院,昆明650092

出　　处：《理化检验（化学分册）》2010年第9期1008-1009,1012,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用高速逆流色谱从独活的乙醇粗提物中分离纯化水合氧化前胡素。用氯仿-甲醇-水(4+4+2)为两相溶剂系统,上相为固定相,下相为流动相。所得下相溶剂经高效液相色谱-紫外检测器测定及高分辨电子轰击质谱和核磁共振氢谱鉴定,为水合氧化前胡素,其纯度为98.3%。High-speed counter-current chromatography（HSCCC） was applied to the separation and purification of the primary ethanol extract of Radix angelicae pubescentis.A two-phase solvent system of chloroform,methanol and water in the ratio of 4 to 4 to 2 was selected,and the upper phase was used as stationary phase and lower phase was used as mobile phase.The solvent of lower phase obtained was determined by HPLC with UV detector,and identified by EI-MS and H1-NMR.The main component of Radix angelicae pubescentis was found to be oxypeucedanin hydrate,with its purify of 98.3%.

关 键 词：高速逆流色谱 独活 水合氧化前胡素 

分 类 号：O657.7[理学—分析化学]