高效毛细管电泳法分离4-羟基-苯异丙胺对映体  

Separation of Enantiomers of 4-Hydroxy-Amphetamine by High Performance Capillary Electrophoresis

作　　者：胡林峰[1] 张裕平[1] 王雪静[1] 荆瑞俊[1] 陈娜[1] 杨胜凯[1] 

机构地区：[1]河南科技学院化学化工学院,河南453003

出　　处：《理化检验（化学分册）》2010年第9期1058-1059,1062,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南科技学院重点科研项目资助基金

摘　　要：在内径100μm的毛细管内,以甲基丙烯酸甘油酯(GMA)和双(六-O-丁烯二酸单酯)-β-环糊精为单体原位聚合制得了手性毛细管电色谱整体柱,采用高效毛细管电泳法拆分了4-羟基-苯异丙胺对映体。考察了背景电解质的pH值、运行电压、柱温、流动相的配比等因素对分离度的影响。在背景电解质pH为4.2、运行电压20 kV、柱温18℃、乙腈-9 mmol.L-1磷酸盐缓冲溶液(60+40)为流动相、在4 min内成功地分离了4-羟基-苯异丙胺对映体,其分离度达到1.56。In-situ polymerization of monomers of glycerol methyl propenoiate（GMP） and bis-（6-O-monoester of butenedioic acid）-β-cyclodextrin was performed in a capillary of bore diameter of 100 μm to prepare the chiral capillary monolithic column,which was used for the HPCE separation of enantiomers of 4-hydroxy-amphetamine.Optimum conditions for the separation were found as follows： ① acidity of background electrolyte： pH 4.2;② temperature of capillary column： 18 ℃;③ separation voltage： 20 kV;④ mobile phase： mixture of acetonitrile and 9 mmol·L^-1 PBS,mixed in the ratio of 60 to 40 by volume.Under the optimized conditions,the enantiomers of 4-hydroxy-amphetamine were satisfactorily separated within 4 min,having value of resolution factor 1.56.

关 键 词：高效毛细管电泳法 4-羟基-苯异丙胺对映体 整体柱 手性分离 双(六-O-丁烯二酸单酯)-β-环糊精 

分 类 号：O657.8[理学—分析化学]