反相高效液相色谱法测定不同产地布渣叶的3种水解黄酮苷元  

RP-HPLC Determination of 3 Flavonoid Aglycone Hydrolyzates of Leaves of Microcos Paniculata L.from Different Origins

作　　者：李坤平[1,2] 潘天玲[2] 周宏兵[2] 李卫民[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]广东药学院药科学院,广州510006

出　　处：《理化检验（化学分册）》2010年第9期1060-1062,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广东省科技计划项目(2006B35605005)

摘　　要：提出了反相高效液相色谱法同时测定布渣叶的3种水解黄酮苷元槲皮素、山奈酚和异鼠李素的含量。采用Kromasil C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇-0.4%（体积分数）磷酸溶液为流动相梯度洗脱,在368 nm波长处,对布渣叶的水解液进行了色谱分离测定。结果表明：槲皮素的质量浓度在1.97-19.7 mg·L^-1,山奈酚在2.02-20.2 mg·L^-1,异鼠李素在2.11-21.1 mg·L^-1时分别与其峰面积呈线性关系。槲皮素、山奈酚和异鼠李素的加标回收率分别为93.0%-99.6%,92.6%-99.8%,92.5%-99.5%,相对标准偏差（n=6）分别为2.48%,2.80%,3.17%。RP-HPLC was applied to the simultaneous determination of 3 flavonoid aglycone hydrolyzates including quercetin,kaempferol and isorhamnetin by hydrolysis of leaves of Microcos paniculata L.from different regions.The hydrolyzed solution of sample was separated by gradient elution with methanol and 0.4%（φ） phosphoric acid solution on Kromasil C18 column（250 mm×4.6 mm,5 μm）,and their contents determined at the wavelength 368 nm.Linear relationships between values of peak area and mass concentration of quercetin,kaempferol and isorhamnetin were kept in ranges of 1.97-19.7 mg·L^-1,2.02-20.2 mg·L^-1,and 2.02-20.2 mg·L^-1,respectively.Tests for recovery and precision were made by standard addition method,giving values of recovery in the range of 93.0%-99.6%,（quercetin）,92.6%-99.8%（kaempferol） and 92.5%-99.5%（isorhamnetin）.Values of RSD′s（n=6） found were 2.48% for quercetin,2.80% for kaempferol and 3.17% for isorhamnetin.

关 键 词：反相高效液相色谱法 布渣叶 槲皮素 山奈酚 异鼠李素 

分 类 号：O652.63[理学—分析化学]