甲型H1N1流感病毒致宿主细胞脱氧核糖核酸氧化损伤的研究  

Study on the DNA oxidation damage of the MDCK cell caused by H1N1 infection

作　　者：郑君德[1] 刘彦明[2] 刘汉欣[1] 高月亭[1] 陈涛[1] 张丽梅[1] 余琳[1] 肖洪广[1] 

机构地区：[1]广州医学院第一附属医院检验科,广州510120 [2]汕头大学附属粤北人民医院检验科,广州510026

出　　处：《中日友好医院学报》2010年第5期289-291,F0004,共4页Journal of China-Japan Friendship Hospital

基　　金：广东省自然科学基金博士科研启动项目(9451018201003654);广东省中医药局建设中医药强省科研课题项目(2008422);广州医学院博士启动基金项目(2008C17)

摘　　要：目的:通过检测DNA损伤标志物8-氧鸟嘌呤脱氧核苷(8-oxo-dG)在甲型H1N1型流感病毒感染MDCK细胞中的表达,初步探讨甲型H1N1型流感病毒诱导细胞凋亡的脱氧核糖核酸(DNA)损伤机制。方法:MDCK细胞培养后,甲型H1N1流感病毒感染继续培养0h、1h、3h、6h、12h、24h、48h,细胞爬片免疫组织化学方法检测各时间点DNA损伤标志物8-oxo-dG的表达。结果:甲型H1N1流感病毒感染后各时间点实验组MDCK细胞8-oxo-dG的表达均显著高于相应的正常对照组(P<0.05),病毒感染各组间两两比较发现各组间差异显著。结论:甲型H1N1流感病毒感染能造成宿主细胞DNA氧化损伤。Objective：To explore the apoptosis mechanism of H1N1 influenza virus infection to MDCK cell by detecting the expression of 8-hydroxy-2＇-desoxyguanosine（8-oxo-dG） as biochemical marker of DNA damage.Methods：MDCK cells were seeded in slide of 48-well plate and infected with H1N1 influenza virus.Immunohistochemistry method was used to investigate 8-oxo-dG expression in MDCK cell respectively after being infected for 0,1,3,6,12,24 and 48 hours.Results：Significant difference of 8-oxo-dG in MDCK cells could be observed between every case-control pair at 0,1,3,6,12,24 and 48 hours;and significant increase of 8-oxo-dG could also be found among the experiment groups（P0.05）.Conclusion：H1N1 influenza virus infection can induce significant DNA oxidative damage.

关 键 词：甲型H1N1流感病毒 脱氧核糖核酸氧化损伤 8-羟基脱氧鸟嘌呤 

分 类 号：R373.13[医药卫生—病原生物学]