5-羟色胺在野百合碱引起大鼠肺动脉高压发病中的作用  

The role of 5-hydroxypytamine in monocrotaline-induced pulmonary hypertension

作　　者：张福琴[1,2] 李志超[1,2] 赵德化[1,2] 黄威权 孙本韬[1,2] 

机构地区：[1]第四军医大学病理生理学教研室 [2]山西医学院电镜室

出　　处：《中国病理生理杂志》1999年第5期462-465,共4页Chinese Journal of Pathophysiology

摘　　要：目的：探讨５－ＨＴ在ＭＣＴ性肺动脉高压中的作用。方法：用免疫组化方法和图象分析技术，测定肺组织中５－ＨＴ及其受体的变化；采用ＭＴＴ法和测微网及钙荧光指示剂Ｆｕｒａ２／Ａｍ测定培养的肺动脉平滑肌细胞生长情况和收缩功能以及细胞内游离Ｃａ２＋浓度。结果：发现ＭＣＴ引起大鼠肺动脉高压时肺组织中５－ＨＴ（１２７４９４±２０７３６／ｍｍ２）及其受体（１４６２４±３０１０／ｍｍ２）阳性细胞数目比对照组（３７２０±１０１８，３４３０±８２１／ｍｍ２）的均显著增加（Ｐ＜００１）。５－ＨＴ能使培养的肺动脉平滑肌细胞增生和收缩这种作用与其引起的Ｃａ２＋内流有关。AIM and METHODS: To explore the effect of 5-hydroxytryptamine (5-HT) in monocrotaline(MCT)-induced pulmonary hypertension, 5-HT and 5-HT receptor ( 5-HTR) in pulmonary tissue was observed by immohistochemistry and image analysis system. Cellular growth, constrictions function and free Ca 2+ in cultured pulmonary artery smooth muscle cells were measured by the methods of MCT, morphometry and calcium fluorescent probe Fura 2-Am. RESULTS:The numbers of 5-HT(127 494±20 736/mm 2) and 5-HTR (14 624±3 010/mm 2) positive cells in pulmonary tissue induced by MCT were increased compared with control (3720±1018, 3430±821/mm 2). 5-HT could induce both cellular growth and contraction of pulmonary arterial smooth muscle cells. Free Ca 2+ in cultured pulmonary arterial smooth muscle cells was significantly increased by 5-HT (242±12 nmol/L) compared with control(95±4 nmol/L).CONCLUSION:Cellular hyperplasia and contraction of pulmonary arterial smooth muscle cells induced by 5-HT were an important mechanism of MCT-induced pulmonary hypertensiom.

关 键 词：野百合碱 大鼠 肺动脉高压 5-羟色胺 

分 类 号：R543.202[医药卫生—心血管疾病]