镉诱导人胚肾细胞金属硫蛋白基因亚型的表达  

Metallothion Isoforms Gene Expression Induced by Cadmium in Human Embryo Kidney Cell

作　　者：常秀丽[1] 金泰廙[1] 陈亮[1] 

机构地区：[1]复旦大学公共卫生安全教育部重点实验室公共卫生学院劳动卫生教研室,上海200032

出　　处：《环境与职业医学》2010年第9期534-537,共4页Journal of Environmental and Occupational Medicine

基　　金：国家973课题项目资助(编号:2002CB512905)

摘　　要：[目的]研究镉对人胚肾细胞(HEK293T)金属硫蛋白(MT)各基因亚型表达的影响。[方法]以10μmol/LCdCl2染毒HEK 293T0、2、4、8、12、16、20、24h,2.5、5、10、20、40μmol/LCdCl2染毒HEK293T24h后,采用MTT法测定HEK 293T增殖活性;采用RT-PCR检测镉对HEK 293T MT-1A、MT-1B、MT-1E、MT-1F、MT-1G、MT-1H、MT-1X及MT-2A mRNA表达的影响。[结果]CdCl2染毒HEK 293T 24h后,经10μmol/LCdCl2诱导后,随时间MT-1A、MT-1F、MT-1G、MT-1H及MT-2A mRNA表达水平均呈先升高后降低趋势。MT-1X mRNA水平在8h明显升高(P<0.05),16h达到峰值并持续到24h。5μmol/L以上CdCl2均可明显抑制HEK293T活力,与对照组相比差异均有统计学意义(P<0.05)。MT-1A、MT-1F、MT-1G、MT-1H及MT-2A mRNA在2.5μmol/LCdCl2诱导表达水平均达到峰值,随着剂量增加,MT-1F、MT-1G、MT-1H维持在较高水平,40μmol/L剂量水平仍明显高于对照组(P<0.05),MT-1A、MT-2A在40μmol/L降低到基础表达水平。MT-1X mRNA表达水平与染毒剂量呈线性正相关(r=0.75,P<0.01)。MT-1X的表达与镉对HEK 293T毒性呈负相关关系(r=-0.88,P<0.01)。[结论]镉可以诱导MT-1F、MT-1G、MT-1H、MT-1X及MT-2A mRNA表达水平明显升高,并表现出特定的剂量-效应和时间-效应关系。[ Objective] To explore gene expression of metallothionein 1（MT-1）isoforms in human embryo kidney cel（lHEK 293T）.[ Methods] Treatment with 2.5, 5, 10, 20, and 40 μmol/L CdCl2 for 24 h, and 10 μmol/L CdCl2 for 0, 2, 4, 8, 12, 16, 20, and 24 h to HEK 293T cell, the cell viability was measured with MTT assay. The expression of MT-1A, MT-1B, MT-1E, MT-1F, MT-1G, MT-1H, MT-1X and MT-2A mRNA was determined in HEK 239T cell after exposure to cadmium by quantitative RT-PCR. [Results] Treatment with different levels of CdCl2 for 24 h significantly reduced the viability in more than 5 μmol/L CdCl2. The diverse expression patterns of these genes（MT-1A, MT-1F, MT-1G, MT-1H, MT-1X and MT-2A） mRNA expression depending on Cd concentration and exposure time was significantly increased after exposure to cadmium（P 0.05）.[ Conclusion] Results indicated that MT-1A, MT-1E, MT-1F, MT-1X, and MT-2A mRNA expression was induced by cadmium, and exhibits dose-effect and dose-course relationship with Cd exposure level.

关 键 词：金属硫蛋白 基因 人胚肾细胞 镉 

分 类 号：R13[医药卫生—劳动卫生]