海洋药用生物系列HPLC化学指纹图谱研究Ⅰ--二色补血草HPLC化学指纹图谱研究  

Study on Series of HPLC Fingerprint Ⅰ: A Research of HPLC Fingerprint for Limonium bicolor

作　　者：张敏[1] 唐旭利[2] 李国强[1] 王长云[1] 管华诗[1] 

机构地区：[1]中国海洋大学医药学院,海洋药物教育部重点实验室,山东青岛266003 [2]中国海洋大学化学化工学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2010年第10期95-99,共5页Periodical of Ocean University of China

基　　金：国家海洋局908专项计划项目(908-01-ST12);国家高技术研究发展计划项目(2007AA091701)资助

摘　　要：建立滨海湿地药用植物二色补血草的HPLC化学指纹图谱。运用DAD-Agilent 1100高效液相色谱仪,色谱条件:Agilent Zorbax XDB C18色谱柱(4.6 mm×250 mm;5.0μm),乙腈-0.1%磷酸水流动相梯度洗脱,流速1.0 mL.min-1,检测波长200 nm,参比波长360 nm,柱温30℃。首次建立的二色补血草HPLC化学指纹图谱包含17个共有峰。方法的精密度、重现性和稳定性试验RSD值均<5.0%。结论:所建立的二色补血草HPLC化学指纹图谱可用于药材的真伪鉴别。To establish HPLC fingerprint of Limonium bicolor.The reference fingerprint of L.bicolor was developed by the HPLC method.The chromatographic condition was described as Agilent XDB C18 column,acetonitrile-0.1% phosphoric acid water with gradient elution as mobile phase,flow-rate 1.0 mL·min^-1,detection wavelength 200 nm and column temperature 30 ℃.Results The reference fingerprint of L.bicolor which included 17 common peaks was built for the first time.The RSD values of the precision,stability and repeatbility tests for the method were less than 5.0%.The reference fingerprint could be used for the identification of L.bicolor.

关 键 词：二色补血草 指纹图谱 HPLC 真伪鉴别 

分 类 号：R284[医药卫生—中药学]