湖南省蔬菜根结线虫发生及种类鉴定  

Species identification and occurrence investigation of root-knot nematodes on vegetables in Hunan Province

作　　者：成飞雪[1,2] 张德咏[2,3] 何明远 程菊娥 肖启明[1] 刘勇[2,3] 

机构地区：[1]湖南农业大学生物安全学院,长沙410128 [2]湖南省植物保护研究所,长沙410125 [3]中南大学研究生院隆平分院,长沙410125

出　　处：《植物保护》2010年第5期128-132,共5页Plant Protection

基　　金：国家自然科学基金青年基金(3080717);行业科技专项(nyhyz07-050);国家"863"计划项目(2006AA10Z401);"十一五"国家科技支撑计划项目(2006BAD17B08;2006BAD08A08)

摘　　要：对湖南省蔬菜主产区的根结线虫病发生与危害程度进行了系统调查,结果显示蔬菜根结线虫病发生普遍,大棚蔬菜发病率高达80%,露地蔬菜发病率也在10%左右。对采自湖南省内10种蔬菜作物上的23个根结线虫种群进行了种类鉴定。采用2龄幼虫测量值、会阴花纹形态特征、PCR法及序列测定相结合的方法进行了鉴定,结果表明南方根结线虫是湖南省蔬菜根结线虫病的优势病原种群,占87%。Systematic investigation on occurrence and damage of vegetable root-knot nematode diseases was con- ducted in Hunan. The results showed that vegetable root-knot nematode diseases in Hunan occurred universally. Nematode-infected plants were found in 80% greenhouses and in 10% fields. Twenty-three samples of root-knot nematode were collected from various vegetables in Hunan. The morphology value of J2 and the perineal pattern of female and the ITS sequences of nematode were employed to identify the samples. The results indicated that Meloidogyne incognita was the dominant species.

关 键 词：根结线虫 种类鉴定 会阴花纹 ITS序列 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]