茶毛虫核型多角体病毒和Bt混剂的配比筛选及药效评价  

Screening for ratio of EpNPV mixed with Bt and evaluation of its control effect

作　　者：唐美君[1] 殷坤山[1] 郭华伟[1] 刘琴[2] 姚惠明[1] 肖强[1] 

机构地区：[1]中国农业科学院茶叶研究所,杭州310008 [2]扬州市农业科学研究院,扬州225007

出　　处：《植物保护》2010年第5期165-167,共3页Plant Protection

基　　金：浙江省科技计划项目(2007C12035);国家科技支撑计划项目(2008BADA5B06);科技部公益基金项目(2005DIA4J052)

摘　　要：在室内条件下采用浸叶法测定了茶毛虫核型多角体病毒（ Euproctis pseudoconspersa nucleopolyhedrovirus, EpNPV）和苏云金杆菌（Bacillus thuringiensis , Bt）及其不同配比对茶毛虫的毒力，结果表明，EpNPV和Bt5种比例混用均表现出不同程度的增效作用，共毒系数在177．3～221．7之间。其中以1．0×10^4PIB／μL EpNPV＋2000IU／μL，Bt混用增效最明显，其毒效是Bt单剂的2．54倍。田间小区试验表明，EpNPV-Bt混剂（1．0×10^4PIB／μL EpNPV＋2000IU／μLBt）对茶毛虫幼虫有优良的防效，显著优于Bt单剂和EpNPV单剂（声〈0．05）。The virulence of EpNPV, Bt and their mixture against the larvae of Euproctis pseudoconspersa was investigated in laboratory. All of the five ratios of EpNPV mixed with Bt showed synergistic effects. The co-toxicity coefficient ranged from 177. 3 to 221. 7. Among the five ratios, 1. 0 ×104 PIB/μL EpNPV mixed with 2 000 IU/μL Bt had the highest synergistic effect, in which the toxicity was 2.54 times higher than that of Bt alone. The field trials demonstrated that EpNPV-Bt （1.0 × 104 PIB/μL EpNPV mixed with 2 000 IU/μL Bt） had a significantly better control effect than both Bt and EpNPV alone（p〈0.05）.

关 键 词：EpNPV Bt增效作用 配比 田间防效 茶毛虫 

分 类 号：S482.39[农业科学—农药学]