半乳糖苷凝集素-7免疫增强功能的体外研究  

In Vitro Study of Enhancing Immune Function by Galectin-7,a Member of Galectins

作　　者：高英[1] 张维娜[1] 何文涛[1] 汪理[2] 周鸿敏[1] 林星光[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,教育部、卫生部器官移植重点实验室,武汉430030 [2]华中科技大学同济医学院附属协和医院胰腺外科,武汉430022

出　　处：《医药导报》2010年第10期1261-1265,共5页Herald of Medicine

基　　金：“973”计划专项基金(基金编号:2009CB-522407)

摘　　要：目的了解半乳糖苷凝集素-7(galectin-7)对不同T细胞亚群增殖的影响,并进一步探讨其免疫治疗方面的应用前景。方法利用不同浓度的重组Galectin-7蛋白在有或无抗CD3和CD28抗体存在时与纯化的T细胞共同培养5 d,流式细胞仪检测T细胞及各亚群的增殖情况。结果 galectin-7能明显诱导T细胞的增殖,在与抗CD3和抗CD28抗体联用时能协同促进T细胞增殖反应,增殖的细胞主要为CD8+T细胞。结论 galectin-7能诱导并促进T细胞的增殖,在体外发挥免疫增强作用,深入探索其作用机制,有望研发新的靶点药物用于肿瘤的临床免疫治疗。Objective To investigate the effect of galectin-7 on proliferation of T lymphocyte subpopulations and clinic application. Methods The purified T lymphocytes were cultured with recombined galectin-7 at different concentrations with or without anti-CD3 and anti-CD28 antibody for 5 days.The proliferation of T lymphocytes and subpopulations was detected by using FACS analysis. Results galectin-7 significantly induced the proliferation of T lymphocytes and enhanced T lymphocyte proliferation,when it was cocultured with anti-CD3 and anti-CD28 antibody.CD＋8 T lymphocytes were the major proliferated T lymphocytes. Conclusion galectin-7 could elicit and promote T lymphocytes proliferation and exert immunological enhancomem in vitro.galectin-7 may become a new target for new drugs development for clinic immunotherapy of carcinoma by studying the mechanisms deeply.

关 键 词：半乳糖苷凝集素-7 增殖 T细胞亚群 

分 类 号：R979.1[医药卫生—药品] R967[医药卫生—药学]