环孢素A治疗前后慢性再生障碍性贫血患者骨髓吲哚胺2,3双加氧酶水平变化  

Expression and significance of IDO in chronic alplastic anemia patients treating by ciclosporin A

作　　者：陈玮[1] 刘宵虹[1] 张伟华[1] 张秀莲[1] 范星火[1] 侯素敏[1] 刘晨[1] 

机构地区：[1]山西医科大学第一医院血液科,太原030001

出　　处：《中国药物与临床》2010年第10期1098-1100,共3页Chinese Remedies & Clinics

基　　金：山西省科技攻关项目(20090311058-5)

摘　　要：目的检测环孢素A治疗前后慢性再生障碍性贫血(AA)患者骨髓单个核细胞中吲哚胺2,3双加氧酶(IDO)的表达情况,探讨IDO在慢性AA发病机制中的作用。方法用反转录-聚合酶链反应(RT-PCR)检测9例初治及环孢素A治疗3个月后慢性AA患者骨髓单个核细胞中IDO mRNA的表达水平,并分析其与中性粒细胞绝对值、网织红细胞相对值、血小板计数及血红蛋白含量相关性。数据分析采用t检验和直线相关分析。结果①初治慢性AA患者骨髓单个核细胞中IDO mRNA表达强度为(0.42±0.10),明显高于对照组[(0.19±0.14),P<0.01]。②环孢素A治疗3个月后慢性AA患者骨髓单个核细胞中IDO mRNA表达强度为(0.19±0.12),明显低于治疗前[(0.42±0.10),P<0.01]。③IDO与中性粒细胞绝对值、网织红细胞相对值、血小板计数及与血红蛋白含量的r值分别为0.459、-0.056、-0.482、0.006(P均>0.05),均无明显线性相关关系。结论在慢性AA患者骨髓单个核细胞中,IDO表达增加,促进色氨酸向犬尿氨酸转化,而色氨酸的耗竭及其代谢产物犬尿氨酸可抑制T淋巴细胞的增殖、诱导T淋巴细胞的凋亡,提示IDO在慢性AA的发病机制中可能起保护作用。Objective To explore the significance of indoleamine 2,3-dioxygenase（IDO） in patients with chronic aplastic anemia（AA） and the role of IDO in the pathogenesis of chronic AA,we examined IDO mRNA expression in bone marrow mononucleated cells in chronic AA patients and evaluated the relationships between IDO mRNA and hemocytogenesis.Methods Heparinized bone marrow samples were obtained from 9 patients with chronic AA without any treatment and after three month treatment by ciclosporin A,the IDO mRNA of bone marrow mononucleated cells in chronic AA was measured by reverse transcriptase PCR.Comparisons between groups were performed with t-test and linear correlation analysis.Results ①Expression of IDO mRNA was higher in chronic AA than in healthy controls [（0.42±0.10） vs（0.19±0.14）,P〈0.01].②The IDO mRNA levels from patients after three months immunosuppressive treatment were significantly decreased compared with the pretherapeutic patients[（0.19±0.12） vs（0.42±0.10）,P〈0.01].③Expression of IDO had no relationship with hemoglobin,platelet,reticulocyte and absolute neutrophil count in patients with chronic AA.Conclusion This suggests that the increased IDO may be a protective factor in patients with chronic AA.

关 键 词：贫血 再生障碍性 双加氧酶类 环孢菌素 基因表达 

分 类 号：R556[医药卫生—血液循环系统疾病]