罗勒挥发油超临界CO_2萃取及GC-MS分析  

Extraction of volatile oil from Ocimum basilicum L.by supercritical CO_2 and GC-MS analysis of the extract

作　　者：周荣[1] 王艳[1] 任吉君[1] 周小梅[1] 

机构地区：[1]佛山科学技术学院园艺系,广东佛山528231

出　　处：《湖南农业大学学报（自然科学版）》2010年第5期585-588,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：广东省农业攻关项目(2007B020712003)

摘　　要：采用超临界CO2萃取技术,利用正交设计,对影响罗勒挥发油萃取工艺参数(萃取压力、萃取温度、萃取时间)以及挥发油成分进行了研究.结果表明,罗勒挥发油超临界CO2萃取工艺参数的最佳组合是:萃取压力为12MPa,萃取温度为45℃,萃取时间为2h,萃取率达0.761%.所获得的挥发油呈黄色半透明状,具芳香味.采用GC-MS对挥发油进行分析,共鉴定出30种化合物,主要成分为萜类物质,占总量的96.64%.相对含量最高的是芳樟醇,占69.83%,其次分别是(+)表-双环倍半水芹烯(6.73%)和1,8-桉叶油素(5.01%).The supercritical CO2 extraction technology was used to extract volatile oil from Ocimum basilicum L.The technical conditions affecting the extraction （pressure,temperature and time） were optimized by using orthogonal design,and the constituents of the volatile oil were studied by analyzingGC/MS.The results showed that the optimal conditions were,using 12 MPa of pressure,at 45 ℃ temperature and extracted for 2 h.Under this conditions,the extraction ratio was 0.761%.GC/MS analysis identified 30 compounds in the volatile oil.The main chemical component was terpenoids,which accounted for 96.64% of the total volatile oil.The relative content of the highest was linalool,containing 69.83%,followed by the （＋）-Epi-bicyclosesquiphellandrene （6.73%） and 1,8-Cineole （5.01%）.

关 键 词：罗勒 挥发油 超临界CO2萃取 气相色谱-质谱法 

分 类 号：O658.2[理学—分析化学]