采收季节与年龄对曼地亚红豆杉主要活性成分的影响  

Effects of gathering season and three age affect on main active components of Taxus madia

作　　者：史小娟[1] 徐志明 付顺华[1] 张新凤[1] 何照斌 杜红亮 

机构地区：[1]浙江农林大学天然药物研究开发中心亚热带森林培育国家重点实验室,浙江临安311300 [2]浙江常山曼地亚红豆杉科技发展有限公司,浙江常山324200

出　　处：《中国中药杂志》2010年第19期2538-2540,共3页China Journal of Chinese Materia Medica

基　　金：浙江省重大科技专项(2008C12020)

摘　　要：目的:揭示采收季节与年龄对曼地亚红豆杉枝叶中紫杉醇(taxol)和10-脱乙酰巴卡亭Ⅲ(10-deacetyl baccatin Ⅲ,10-DABⅢ)含量的影响,为曼地亚红豆杉适时采收提供依据。方法:2009年3月26日至2010年1月26日每隔2个月采集经标记的25株三年生植株和25株五年生植株枝叶,采用HPLC测定不同年龄与生长季节曼地亚红豆杉枝叶中紫杉醇和10-DABⅢ的含量。结果:不同采收年龄的曼地亚红豆杉紫杉醇和10-DABⅢ含量存在显著差异,五年生植株显著高于三年生植株;不同采收季节的曼地亚红豆杉紫杉醇和10-DABⅢ含量也存在显著差异,全年中5月份的含量最高,紫杉醇最高含量为0.56mg.g-1,10-DABⅢ最高含量为0.32mg.g-1。结论:曼地亚红豆杉枝叶在5月份采收最佳,而采收年龄尚需根据主要活性成分及生物量积累规律作进一步研究。Objective：To reveal the influence of harvest season and tree age on the content of taxol and 10-DAB Ⅲ, and provide the basis for the harvest time of Taxus madia. Method： Branches and leaves of the labeled 25 three-year-old plants and 25 five-year-old plants were collected every two months from March 26,2009 to January 26,2010. Taxol and 10-DAB Ⅲ content of different age and growth season of Taxus in branches were determined by HPLC. Result： Taxol and 10-DAB Ⅲ content were significantly different in different harvesting age. The content of five-year-old plants was significantly higher than that of three-year-old plants. Taxol and 10-DAB Ⅲ contents were significantly different in different harvesting season, and the highest content of taxol and 10-DAB Ⅲ was 0.56, 0.32 mg·g-1, respectively, in May. Conclusion： The May is the suitable harvest season for T. madia, but the suitable harvest age need further study which according to the main active component and biomass accumulation.

关 键 词：曼地亚红豆杉 紫杉醇 10-DABⅢ 采收季节 采收年龄 

分 类 号：S567.19[农业科学—中草药栽培]