联用Plackett-Burman与Box-Behnken设计控制青黛制备过程中靛玉红的生成  

United Plackett-Burman and Box-Behnken design to control formation of indirubin in process of preparing indigo naturalis

作　　者：刘泽玉[1] 苏柘僮[1] 杨明[1,2] 邹文铨[3] 

机构地区：[1]成都中医药大学,四川成都610000 [2]江西中医学院中药现代制剂教育部重点实验室,江西南昌330004 [3]四川大学,四川成都610000

出　　处：《中国中药杂志》2010年第19期2551-2555,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30772784);<中国药典>2010版一部标准研究

摘　　要：目的:筛选出青黛炮制过程中显著影响靛玉红生成的因素,优化最佳水平组合,确定生成靛玉红的最佳工艺。方法:以鲜叶产靛玉红的质量分数(mg·g-1)为指标,采用Plackett-Burman设计,Box-Behnken设计效应面分析等统计学方法,筛选出显著影响因素及最佳水平组合。结果:青黛的浸泡、打靛工艺确定为浸泡前不除蜡、浸泡液pH7、溶剂用量-鲜叶质量(mL∶g)15、浸泡不避光、浸泡48h、温度60℃、通气时间180min、加氨水调节打靛前pH至10.5。结论:系统考察了青黛炮制过程中各个影响因素对产生的有效成分靛玉红的影响,使工业化生产控制靛玉红的生成变为现实,为进一步研究青黛炮制原理奠定了基础。Objective：To screen the factors that affect indirubin-generated significantly in the process of preparing indigo naturalis, optimize level combination and determine the optimum technology for indirubin-generated. Method： Using concentration of indirubin（mg·g-1）that generated by fresh leaf as an index, Plackett-Burman design, Box-Behnken design response surface analysis as the statistical method, we screened the significantly influencing factors and the optimal level combination. Result： The soaking and making indirubin process in preparing indigo naturalis was identified as the wax is not removed before immersion with immersion pH 7, solvent volume-leaf weight （mL∶g）15, soaked not avoided light, soaking 48 h, temperature 60 ℃, ventilation time of 180 min, and added ammonia water to adjust pH to 10.5. Conclusion： The soaking and making indirubin process in preparing indigo naturalis is optimized systematically. It clarify the various factors on the impact of the active ingredient indirubin which controlled by industrialized production become reality in the process of preparing indigo naturalis, at the same time, it lay the foundation for processing principle of indigo naturalis.

关 键 词：靛玉红产量 PLACKETT-BURMAN设计 BOX-BEHNKEN设计 最佳工艺 

分 类 号：R284[医药卫生—中药学]