千里光中的生物碱类成分  

Alkaloids from Senecio scandens

作　　者：谭道鹏[1] 陈莹[1] 季莉莉[2,3] 侴桂新[2,3] 王峥涛[1,2,3] 

机构地区：[1]中国药科大学中药学院,江苏南京210009 [2]上海中医药大学中药标准化教育部重点实验室,上海201203 [3]上海中药标准化研究中心,上海201203

出　　处：《中国中药杂志》2010年第19期2572-2575,共4页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI14B01)

摘　　要：目的:研究千里光Senecio scandens中的生物碱类化学成分。方法:采用硅胶和凝胶柱色谱进行分离,根据化合物理化性质和光谱数据鉴定其结构。采用MTT法对所得到的化合物进行原代小鼠肝细胞毒性检测。结果:分离得到6个化合物,分别为:阿多尼弗林碱(1),7-angeloylturneforcidine(2),大麦芽碱(3),1,3,6,6-四甲基-5,6,7,8-四氢异喹啉-8-酮(4),4-(吡咯烷-2-酮基)-苯基乙酸(5),4-吡咯烷基-苯基乙酸(6)。结论:化合物6为新天然产物,化合物3,4为首次从该属植物中分离得到,化合物2,5为首次从该植物中分离得到。毒性筛选结果表明,化合物1对原代小鼠肝细胞有细胞毒作用,其他化合物未表现出细胞毒作用。Objective：To investigate the alkaloids from Senecio scandens. Method： Compounds were isolated with silica gel and Sephadex LH-20 column chromatography and their structures were determined by spectral analysis and chemical evidence. The hepatic cytotoxicity of isolated compounds was tested by MTT method in vitro. Result： Six alkaloids were obtained and identified as adonifoline （1）, 7-angeloylturneforcidine （2）, hordenine （3）, 1, 3, 6, 6-tetramethyl-5, 6, 7, 8-tetrahydro-isoquinolin-8-one （4）, 4-（pyrrolidin-2-one）-phenyl acetic acid （5）, （4-pyrrolidinophenyl） acetic acid （6）. Conclusion： Compound 6 is a new natural product, compounds 3, 4 were obtained from the genus Senecio for the first time, compounds 2, 5 were obtained from this plant for the first time. Compound 1 showed significant growth inhibitory effect against hepatocyte at 100 μmol·L-1.

关 键 词：千里光 生物碱 化学成分 毒性 

分 类 号：R284[医药卫生—中药学]