益气清瘟解毒合剂拆方对流感病毒FM_1感染小鼠血清炎性细胞因子的影响  

Influence of separate components of Yiqi Qingwen Jiedu mixture to serum inflammatory cytokines of mice infected with influenza virus FM_1

作　　者：徐红日[1] 王成祥[1] 王惠芳[2] 姜良铎[1] 刘清泉[1] 张靖[2] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]解放军防化研究院,北京102205

出　　处：《中国中药杂志》2010年第19期2599-2604,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873254)

摘　　要：目的:动态观察益气清瘟解毒合剂拆方辛温解表法、辛凉解表法、清热解毒法、益气法(以下简称四法)对流感病毒感染小鼠血清炎性细胞因子的影响,探讨四法在对抗流感免疫损伤及其修复方面的作用机制。方法:建立流感病毒亚洲甲型鼠肺适应株FM1感染小鼠模型,采用ELISA法,在FM1感染的不同时相,动态观察四法对小鼠血清TNF-α,IL-6,IL-1,IFN-γ,IL-105种炎性细胞因子含量的影响。结果:FM1感染小鼠血清TNF-α,IL-6,IL-1,IFN-γ的含量显著增加,而IL-10的含量在感染后第1天低于正常空白组,在感染后3～7d明显高于正常组。四法中辛温解表法在FM1感染后第5天同时显著降低血清TNF-α,IL-6,IL-1的含量,并在感染后第3天及第7天,明显增加血清IL-10的含量,在一定程度上具有抑制免疫损伤的作用。辛凉解表法在FM1感染后5～7d,显著降低血清TNF-α和IL-6的含量,虽在感染后第3天,可能升高血清IL-1的含量,而在感染后第7天又明显降低IL-1的含量,同时在感染后第1～3天及第7天显著升高IL-10的含量,具有一定的抗炎性损伤的作用。清热解毒法在FM1感染后3～5d及第7天同时降低血清TNF-α,IL-6,IL-1的含量,在感染后各时相均显著升高IL-10的含量,具有较强的抑制及修复炎性损伤的作用。益气法在FM1感染后3～7d,显著降低血清TNF-α和IL-6的含量,虽在感染后第3天,可能升高血清IL-1的含量,但在感染后第7天又明显降低IL-1的含量,并且在感染后3～5d及第7天,明显增加IL-10的含量,具有一定的对抗炎性损伤的作用。辛凉解表法在FM1感染后第3天,显著增加血清IFN-γ的含量,而清热解毒法与益气法在FM1感染后1～3d及第7天明显升高血清IFN-γ的含量,具有一定的抗病毒及抑制免疫损伤的作用。结论:中药能通过纠正炎性细胞因子的失衡对抗机体的免疫损伤,并促进损伤的修复,对机体具有一定的保护作用。Objective：To observe dynamically the influence of 4 parts of forming of YQJM（Yiqi Qingwen Jiedu mixture） （referred to as 4 parts of forming）including the methods of relieving superficies with acrid-warm, relieving superficies with acrid-cold, clearing away heat and poison and replenishing Qi to serum inflammatory cytokines of the model mice infected with influenza virus. And to discuss the mechanism of 4 parts of forming of anti-influenza immune injury and restoration. Method： Made the model with the mice infected by FM1 influenza infection, used ELISA method, observed dynamically the influence of four methods on the level of serum TNF-α, IL-6, IL-1, IFN-γ and IL-10 inflammatory cytokines. Result： The level of serum TNF-α, IL-6, IL-1 and IFN-γ of mice infected by FM1 significantly increased, while the level of serum IL-10 was lower than the control group on the first day of infection, but the levels were much higher than the control group in 3 to 7 days after infection. The method of relieving superficies with acrid-warm significantly decreased the levels of serum TNF-α, IL-6, IL-1 on the 5th day after infection, and significantly increased the levels of serum IL-10 on the 3rd and 7th day after infection. The method could inhibit the immune injury to some extent. The method of relieving superficies with acrid-cold decreased the levels of serum TNF-α and IL-6 in 5 to 7 days after infection, increased the level of serum IL-1 on the 3rd day after infection, decreased the level of serum IL-1 on the 7th day after infection, significantly increased the levels of serum IL-10 in 1 to 3 days and on the 7th day after infection. The method could be against inflammatory injury. The method of clearing away heat and poison decreased the levels of serum TNF-α, IL-6, IL-1 after infection in 3 to 5 days and on the 7th day, and significantly increased IL-10 each time after infection. It exhibited more strong inhibition of inflammatory injury and repair. The method of replenishing Qi significantly decreased the

关 键 词：益气清瘟解毒合剂 拆方 流感病毒 血清 炎性细胞因子 

分 类 号：R285.5[医药卫生—中药学]