依达拉奉注射液对AD大鼠学习记忆和海马内APP mRNA表达的影响  

Effects of Edaravone on Learning and Memory Ability and the Mrna Expression of β-Amyloid Precursor Protein in the Hippocampus of Alzheimer's Disease Model Rats

作　　者：李雷[1] 于婷[2] 辛勤[1] 徐旭东[1] 

机构地区：[1]济宁医学院,山东济宁272113 [2]济宁市第一人民医院,山东济宁272111

出　　处：《中国现代应用药学》2010年第9期777-781,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的观察依达拉奉注射液对老年性痴呆(AD)模型大鼠学习记忆功能和海马内APP mRNA表达的影响。方法 44只大鼠随机分为空白对照组12只,造模组12只,依达拉奉低剂量干预组(干预Ⅰ组)10只,依达拉奉高剂量干预组(干预Ⅱ组)10只。用腹腔注射D-半乳糖和灌胃AlCl3法建立AD模型,从实验开始第9周开始,干预Ⅰ组和干预Ⅱ组分别于大鼠尾静脉注射依达拉奉3 mg.kg-1.d-1和6 mg.kg-1.d-1,造模组和对照组分别注射等量生理盐水。实验开始12周后以Morris水迷宫实验检测学习记忆能力,以RT-PCR法检测大鼠脑内海马APP mRNA的表达。结果依达拉奉能显著改善AD模型大鼠的学习记忆障碍(P<0.05),依达拉奉干预Ⅰ组和干预Ⅱ组大鼠脑内海马内APP mRNA表达的水平亦较造模组明显降低(P<0.05)。结论依达拉奉注射液对AD模型大鼠的学习记忆障碍有明显的改善作用。OBJECTIVE To observe the effects of edaravone on learning and memory ability and the mRNA expression of β-amyloid precursor protein（APP） in the hippocampus of Alzheimer’s disease（AD） model rats.METHODS Forty-four rats were randomly divided into 4 groups： blank control group（12）,AD model group（12）,edaravone low-dosage group（10） and edaravone high-dosage group（10）.AD model was made by injection in peritoneum of D-galactose and administration of aluminum chloride.In the ninth week,low-dosage group and high-dosage group were injected respectively in caudal vein with edaravone 3 mg.kg-1.d-1 and 6 mg.kg-1.d-1;control group and model group were injected with equal volume normal saline.After 12 weeks,Morris water maze was used to measure the learning and memory ability,and RT-PCR was used to assay the mRNA expression of APP in the hippocampus.RESULTS Edaravone can significantly improve the disorder of learning and memory ability of AD model rats（P0.05）.The APP mRNA expression levels were significantly lower in low-dosage group and high-dosage group compared with model group（P0.05）.CONCLUSION Edaravone can significantly improve the disorder of learning and memory ability of AD model rats.

关 键 词：尔茨海默病 依达拉奉 实验研究 

分 类 号：R965.2[医药卫生—药理学]