HPLC测定人血浆中霉酚酸及其代谢物浓度  

Determination of Mycophenolic Acid and its Metabolins in Human Plasma by RP-HPLC

作　　者：赵强[1,2] 高峰 

机构地区：[1]山西医科大学公共卫生学院,太原030001 [2]解放军305医院,北京100017 [3]总参警卫局卫生保健处,北京100017

出　　处：《中国现代应用药学》2010年第9期836-840,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立反相高效液相色谱法,同时测定人血浆中霉酚酸（mycophenolic acid,MPA）、酚化葡萄糖醛麦考酚酸（phenol glucuronide metabolite,MPAG）、酰基化MPAG（acyl-MPAG,AcMPAG）的浓度。方法用蛋白沉淀法对样品进行处理。固定相为Zorbax Eclipse XDB C18柱（4.6 mm×250 mm,5μm）,流动相：甲醇：20 mmol-L-1 NaH2PO4（用20%磷酸调至pH 3.0）为55∶45,流速：1.2 mL-min-1,检测波长：304 nm,柱温：45℃。结果 MPA、MPAG、AcMPAG浓度在0.2~50μg-mL-1（r=0.999 7）、2.8~531μg-mL-1（r=0.999 9）、0.3~24μg-mL-1（r=0.999 4）内呈良好的线性关系。MPA及其代谢物的绝对回收率均大于80%,MPA、MPAG、AcMPAG的相对回收率分别为94.0%~101.4%,98.4%~101.9%和96.1%~104.2%。日内及日间RSD远低于15%。结论采用反相高效液相色谱法测定人血浆中MPA、MPAG、AcMPAG的浓度和进行药物代谢动力学研究,方法灵敏度高、重复性强。OBJECTIVE RP-HPLC method detection for simultaneous determination of Mycophenolic acid（MPA）,its phenol glucuronide metabolite（MPAG） and acyl-MPAG（AcMPAG） in human plasma was established.METHODS The plasma samples were prepared with protein-precipitating reagent,and the supernatant was eluted on Zorbax column（250 mm×4.6 mm,5 μm） with 20 mmol-L-1 NaH2PO4 buffer（pH 3.0,adjusted with 20% phosphoric acid） and methanol（45∶55） at 304 nm.The column temperature was 45 ℃,and the flow rate was 1.2 mL-min-1.RESULTS The assay was linear within the range of 0.2-50 μg-mL-1 for MPA（r=0.999 7）,2.8-531 μg-mL-1 for MPAG（r=0.999 9）,and 0.3-24 μg-mL-1 for AcMPAG（r=0.999 4）.Mean absolute recovery of MPA and its metabolites was more than 80%.The average recoveries of MPA,MPAG and AcMPAG were 94%.0–101.4%,98.4%–101.9% and 96.1%–104.2 %,respectively.The RSD of within-day and between-day were all lower than 15%.CONCLUSION The method described is sensitive,reproducible,and will be useful in TDM or pharmacokinetic studies of MPA.

关 键 词：霉酚酸 酚化葡萄糖醛麦考酚酸 酰基化MPAG 反相高效液相色谱法 血浆浓度 

分 类 号：R969.11[医药卫生—药理学]